6533b7d1fe1ef96bd125c262
RESEARCH PRODUCT
Solvent-free microwave-assisted extraction of polyphenols from olive tree leaves: Antioxidant and antimicrobial properties
Francisco J. BarbaGiancarlo CravottoSelin ŞAhinJosé M. LorenzoAyşe Seher Birteksöz TanFarid ChematRuya Samlisubject
Antioxidantmedicine.medical_treatment[SDV]Life Sciences [q-bio]Pharmaceutical ScienceAntioxidantsAnalytical Chemistrychemistry.chemical_compoundDrug Discovery[SDV.IDA]Life Sciences [q-bio]/Food engineeringAntimicrobial; Antioxidant; Oleuropein; Olive leaves; Optimization; Solvent-free microwave extraction; Organic ChemistryOlive leavesMicrowaves04 agricultural and veterinary sciences040401 food scienceAnti-Bacterial AgentsChemistry (miscellaneous)Molecular MedicineAntioxidantAntibacterial activityOptimizationStaphylococcus aureusMicrobial Sensitivity TestsArticlelcsh:QD241-4410404 agricultural biotechnologyOlive leaflcsh:Organic chemistryOleuropeinOleaStaphylococcus epidermidismedicine[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process EngineeringResponse surface methodologyPhysical and Theoretical ChemistryOleuropeinolive leaves; solvent-free microwave extraction; oleuropein; antioxidant; antimicrobial; optimizationChromatographyPlant ExtractsExtraction (chemistry)Organic ChemistryPolyphenolsolive leaves;solvent-free microwave extraction;oleuropein;antioxidant;antimicrobial;optimizationPlant LeaveschemistryPolyphenolYield (chemistry)Solvent-free microwave extractionSolventsAntimicrobialNeural Networks Computerdescription
International audience; Response surface methodology (RSM) and artificial neural networks (ANN) were evaluated and compared in order to decide which method was the most appropriate to predict and optimize total phenolic content (TPC) and oleuropein yields in olive tree leaf (Olea europaea) extracts, obtained after solvent-free microwave- assisted extraction (SFMAE). The SFMAE processing conditions were: microwave irradiation power 250-350 W, extraction time 2-3 min, and the amount of sample 5-10 g. Furthermore, the antioxidant and antimicrobial activities of the olive leaf extracts, obtained under optimal extraction conditions, were assessed by several in vitro assays. ANN had better prediction performance for TPC and oleuropein yields compared to RSM. The optimum extraction conditions to recover both TPC and oleuropein were: irradiation power 250 W, extraction time 2 min, and amount of sample 5 g, independent of the method used for prediction. Under these conditions, the maximal yield of oleuropein (0.060 +/- 0.012 ppm) was obtained and the amount of TPC was 2.480 +/- 0.060 ppm. Moreover, olive leaf extracts obtained under optimum SFMAE conditions showed antibacterial activity against S. aureus and S. epidermidis, with a minimum inhibitory concentration (MIC) value of 1.25 mg/mL.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-06-24 |