A 588-gene microarray analysis of the peripheral blood mononuclear cells of spondyloarthropathy patients

6533b7d1fe1ef96bd125cc63

RESEARCH PRODUCT

A 588-gene microarray analysis of the peripheral blood mononuclear cells of spondyloarthropathy patients

Eric Veys Feng Huang J G Kuipers Wen Chan Tsai D.l. Baeten Jieruo Gu Momiao Xiong J. Kalsi Dafna D. Gladman Elisabeth Märker-hermann Leslie J. Crofford Yeong Wook Song Walter P. Maksymowych H. Deister Markus Rihl D T Y Yu F De Keyser M. Bannai N. Seta

subject

Adult Genetic Markers Male CCR1 Receptors CXCR4 Adolescent Spondyloarthropathy Arthritis Peripheral blood mononuclear cell Arthritis Rheumatoid Psoriatic arthritis Rheumatology medicine Humans Spondylitis Ankylosing Pharmacology (medical)Aged Oligonucleotide Array Sequence Analysis Reverse Transcriptase Polymerase Chain Reaction business.industry Janus kinase 3 Arthritis Psoriatic Synovial Membrane MNDA Interleukin DNA Middle Aged medicine.disease Antigens Differentiation Chemokine CXCL12 Immunology Leukocytes Mononuclear Female business Chemokines CXC

description

OBJECTIVES: To identify genes which are more highly expressed in the peripheral blood mononuclear cells (PBMC) of patients with spondyloarthropathy (SpA), rheumatoid arthritis (RA) and psoriatic arthritis (PsA), in comparison to normal subjects. METHODS: A 588-gene microarray was used as a screening tool to select a panel of such genes from PBMC of these subjects and of normal subjects. Results were then validated by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: The following genes were more highly expressed in arthritis patients than in normal subjects: macrophage differentiation marker MNDA (myeloid nuclear differentiation antigen), MRP8 and MRP14 (migratory inhibitory factor-related proteins); signalling molecules JAK3 (janus kinase 3) and MAP kinase p38 (mitogen-activated protein kinase); receptors TNFR2/p75, C-C-chemokine receptor type 1 (CCR1), C-X-C-chemokine receptor type 4 (CXCR4) and integrin beta1; and the cytokines/chemokines interleukin (IL) 1beta and IL-8. Expression of CXCR4 was unexpectedly high among all arthritis subjects. Using RT-PCR, ELISA and immunohistology, expression of stromal cell-derived factor 1 (SDF-1) was demonstrated in arthritis joints. CONCLUSIONS: The CXCR4/SDF-1 is a potential pro-inflammatory axis for RA, PsA and SpA

year	journal	country	edition	language
2002-01-01	Rheumatology (Oxford, England)

10.1093/rheumatology/41.7.759 https://doi.org/10.1093/rheumatology/41.7.759