6533b7d1fe1ef96bd125cdac
RESEARCH PRODUCT
Lung fixation for the preservation of air spaces.
Rodolfo H. AcostaAngelika Thomas-semmPeter BlümlerStefan Reusssubject
Pulmonary and Respiratory MedicineTissue FixationIsotonic salineClinical BiochemistryIn Vitro TechniquesSuctionHeliumRats Sprague-DawleyFixativesMiceLow vacuumFormaldehydemedicineAnimalsRespiratory systemMolecular BiologyFixation (histology)LungInhalationChemistryAirSyringesStructural integrityAnatomyrespiratory systemMagnetic Resonance ImagingRatsMice Inbred C57BLPulmonary Alveolimedicine.anatomical_structureAlcoholsMicroscopy Electron ScanningLung tissuedescription
The procedures for the fixation of entire lungs of small rodents are presented together with various techniques used to verify the structural integrity of the lung tissue. To achieve this, the lungs were dissected out from rats and mice killed by ether overdose. The specimens were rinsed with isotonic saline and fixation solution under low vacuum conditions. After fixation, they were dried using alcohol and stored in a noncollapsed state (i.e., state of inhalation). Light and scanning electron microscopy as well as magnetic resonance imaging using hyperpolarized 3He were employed to verify the intact state on interalveolar septa and walls of smaller bronchi as well as accessibility of the air spaces.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2004-02-18 | Experimental lung research |