Kinetics of the lipoperoxyl radical-scavenging activity of indicaxanthin in solution and unilamellar liposomes

6533b7d1fe1ef96bd125cdb8

RESEARCH PRODUCT

Kinetics of the lipoperoxyl radical-scavenging activity of indicaxanthin in solution and unilamellar liposomes

Daniela Butera Carla Gentile Luisa Tesoriere M. A. Livrea Mario Allegra

subject

Indicaxanthin membranes radical scavenger liposomes Lipid Peroxides Antioxidant 1 2-Dipalmitoylphosphatidylcholine Pyridines medicine.medical_treatment Radical Lipid Bilayers alpha-Tocopherol Amidines Context (language use)In Vitro Techniques Biochemistry Antioxidants Lipid peroxidation chemistry.chemical_compound Lipid oxidation Suspensions Phosphatidylcholine Nitriles medicine Organic chemistry Liposome Dose-Response Relationship Drug Molecular Structure Methanol Drug Synergism General Medicine Free Radical Scavengers Betaxanthins Solutions Kinetics chemistry Linoleic Acids Liposomes Phosphatidylcholines Solvents Lipid Peroxidation Indicaxanthin Azo Compounds Oxidation-Reduction Nuclear chemistry

description

Abstract The reaction of the phytochemical indicaxanthin with lipoperoxyl radicals generated in methyl linoleate methanol solution by 2,20-azobis(2,4-dimethylvaleronitrile), and in aqueous soybean phosphatidylcholine unilamellar liposomes by 2,20-azobis(2- amidinopropane)hydrochloride, was studied. The molecule acts as a chain-terminating lipoperoxyl radical scavenger in solution, with a calculated inhibition constant of 3.63 £ 105M21 s21, and a stoichiometric factor approaching 2. Indicaxanthin incorporated in liposomes prevented lipid oxidation, inducing clear-cut lag periods and decrease of the propagation rate. Both effects were concentration-dependent, but not linearly related to the phytochemical concentration. The consumption of indicaxanthin during liposome oxidation was remarkably delayed, the lower the concentration the longer the time-interval during which it remained in its native state. Indicaxanthin and a-tocopherol, simultaneously incorporated in liposomes, exhibited cooperative antioxidant effects and reciprocal protective interactions. The extent of synergism decreased at the increase of the ratio (indicaxanthin)/(a-tocopherol). A potential antioxidant mechanism of indicaxanthin is discussed in the context of the chemistry of the molecule, and of the possible reactivity of a short-lived intermediate. Keywords: Indicaxanthin, phytochemical, lipid oxidation, nutritional value

year	journal	country	edition	language
2007-03-17

10.1080/10715760601026614 http://hdl.handle.net/10447/5527