Quality control of commercial mustard by thin-layer chromatography

6533b7d2fe1ef96bd125df83

RESEARCH PRODUCT

Quality control of commercial mustard by thin-layer chromatography

Constantin Măruţoiu Letiţia Oprean Olivia-florena Măruţoiu Cristian Tigae Maria-loredana Soran Monica Carmen Goncea

subject

Chromatography biology Silica gel Clinical Biochemistry Extraction (chemistry)Sinapis Iodine vapor biology.organism_classification Biochemistry Thin-layer chromatography Analytical Chemistry Ammonia chemistry.chemical_compound chemistry Distilled water

description

Horseradish samples ( Cochlearis armoracia L. ) and mustard samples ( Sinapis alba L. ) were extracted with 70:30 ( v/v ) methanol-water in accordance with ISO method 91671. Samples of commercial mustard with and without horseradish were submitted to the same extraction procedure. Separation of the active compounds from these samples were performed on silica gel 60 plates with preconcentration zone and on silica gel F 254 high-performance thin-layer chromatography plates, with iso -propanol-25% ammonia, 90 + 10 ( v/v ), containing different volumes of distilled water (1, 2, 3, or 5 parts), as mobile phases. After development the compounds were visualized in UV light at λ = 254 nm or by exposure to iodine vapor.

year	journal	country	edition	language
2005-08-01	Journal of Planar Chromatography – Modern TLC

https://doi.org/10.1556/jpc.18.2005.4.5

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