6533b7d2fe1ef96bd125e249

RESEARCH PRODUCT

Comparative studies on the detection of benzodiazepines in serum by means of immunoassays (FPIA).

subject

description

Serum was tested for benzodlazeplnes by fluorescence polarlzatlon Immunoassay (FPIA) on Abbott's ADx system uslng the benzodlazeplne serum reagents (Benzo S) and the benzodlazeplne urine reagents (Benzo U) after pretreatment of speclmens by means of acetone preclpltatlon. The followlng sera were included for comparing the two methods: negatlve sera spiked with varlous benzodlazeplnes; 80 sera randomly selected out of a total of 8654 serum specimens from Impalred drlvers; and blood speclmens from Indlvlduals who stated that they had taken benzodlazeplnes. The different benzodlazeplnes were added to serum st concentratlons of 25, 75, and 300 ng/mL. The low-dose benzodlazeplnes flunltrazepam and trlazolam were addltlonally tested at serum concentratlons of 10 ng/mL. Because of the better reproducibility and the shlft of the dynamlc range to lower concentrations, the Benzo S assay was found to be more sensltlve than the Benzo U assay after acetone preclpltatlon. The direct ADx benzodlazeplne serum assay has clear advantages over the acetone precipitation method, especlally wlth regard to therapeutlc concentrations and for the detectlon of the highly potent and low-dose benzodlazeplnes flunltrazepam and trlazolam.