6533b7d2fe1ef96bd125f5f6
RESEARCH PRODUCT
An immunoassay for terbutryn using direct hapten linkage to a glutaraldehyde network on the polystyrene surface of standard microtiter plates.
Iris HartmannWolfram BaumannHeike HolthuesUrsula Pfeifer-fukumurasubject
PolymersSurface PropertiesEnzyme-Linked Immunosorbent Assayengineering.materialBiochemistrySensitivity and Specificitychemistry.chemical_compoundMicrotiter plateCoatingmedicineChromatographymedicine.diagnostic_testHerbicidesTriazinesNetwork onAntibodies MonoclonalchemistryCovalent bondGlutaralImmunoassayCalibrationengineeringPolystyrenesGlutaraldehydePolystyreneHaptenHaptensdescription
2-Aminobutylamino-4-ethylamino-6-isopropylamino-1,3,5-triazine (ABA-atrazine) has been synthesized and used as a coating hapten in an immunoassay with a monoclonal antibody against terbutryn. Coating was achieved by covalently linking ABA-atrazine to a glutaraldehyde polymer network directly bound to the polystyrene surface of a standard 96-well microtiter plate. The assay was carefully optimized. In particular, the coating hapten concentration had a strong effect on the ELISA sensitivity. By including a pre-incubation step a low test midpoint (IC50-value) of 0.130 microg L(-1) was achieved. As far as we are aware this is the most sensitive ELISA for terbutryn yet reported. The coating-hapten-format presented is proposed as generally applicable, because the glutaraldehyde-modified microtiter plate surface enables stable immobilization of a broad variety of coating haptens.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2001-12-01 | Fresenius' journal of analytical chemistry |