6533b7d3fe1ef96bd125fe57
RESEARCH PRODUCT
Evaluation and elimination of the “blank bias error” using the H-point standard addition method
Jorge Verdú-andrésFrancisco Bosch-reigPilar Campíns-falcósubject
AnalyteChemistryAnalytical chemistryTitan yellowBiochemistryBlankBiuret testAnalytical ChemistryAbsorbancechemistry.chemical_compoundReagentStandard additionCalibrationEnvironmental ChemistrySpectroscopydescription
Abstract The basis of the H-point standard addition method in the cases where the analyte determination requires the use of an absorbent reagent is considered. The method evaluates and eliminates the blank bias error present in such procedures by using the absorbance increment at two selected wavelengths as the analytical signal of the calibration graphs. Three different determinations were tested: the determination of proteins with the biuret method, thorium with thoron and of magnesium with Titan Yellow. These procedures are better described by the proposed method than by the traditional approach using absorbance values against reagent blank.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1992-12-01 | Analytica Chimica Acta |