6533b7d3fe1ef96bd125fee5
RESEARCH PRODUCT
Protein transport through gold-coated, charged nanopores: Effects of applied voltage
Salvador MafePieter StroeveKyoung-yong ChunPatricio Ramirezsubject
biologyChemistryAnalytical chemistryGeneral Physics and AstronomyFluxTransport proteinNanoporeIsoelectric pointChemical engineeringIonic strengthbiology.proteinPhysical and Theoretical ChemistryBovine serum albuminPolymeric membraneVoltagedescription
The flux of bovine serum albumin and bovine hemoglobin through charged nanopores inside polymeric membranes is analysed as a function of the applied voltage to the nanopore surface, the solution ionic strength and pH. The electrostatic interaction of the protein with the nanopore surface gives low transport rates except at the protein isoelectric point and the minimum of the effective, voltage-induced nanopore charge. This electrostatic sieving effect allows for the separation of proteins with similar molecular weights.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-02-01 | Chemical Physics Letters |