6533b7d3fe1ef96bd12609ff
RESEARCH PRODUCT
Identification of bound alcohols in soil humic acids by gas chromatography-mass spectrometry
Eric LichtfouseGersende BerthierTorren M. PeakmanSen Dousubject
[SDE] Environmental SciencescampesterolKOH hydrolysisstanols[SDV]Life Sciences [q-bio]TLCFatty alcoholBrassicasterolAlkaline hydrolysis (body disposal)chemical degradation[SDV.SA.SDS]Life Sciences [q-bio]/Agricultural sciences/Soil study010402 general chemistryMass spectrometry01 natural sciencescomplex mixtureskerogenchemistry.chemical_compoundsterolstigmasterolOrganic chemistrySpectroscopyChromatographyhuminChemistryhumic substancesamyrin010401 analytical chemistrybrassicasterolcholesterolGeneral MedicineAtomic and Molecular Physics and OpticsThin-layer chromatography0104 chemical sciences[SDV] Life Sciences [q-bio]CHIMIE ANALYTIQUEsitosteroln-alkanols[SDE]Environmental SciencesHumintriterpenoidGas chromatographyGas chromatography–mass spectrometryGC-MSfatty alcoholdescription
International audience; Humic acids are complex, partly macromolecular, yellow-brownish substances occurring in soils, waters and sediments. In order to shed some light on their molecular structure, crop humic acids were cleaved by alkaline hydrolysis (KOH). The products were fractionated by thin layer chromatography to give mono-alcohols which were analysed as acetate derivatives by gas chromatography coupled to mass spectrometry. Linear alcohols, sterols, stanols and plant-derived triterpenoid alcohols were identified by co-injection of pure standards and by comparison with literature data. These findings imply that alcohols could have been incorporated into the humic matrix by esterification with carboxylic acids. Furthermore, the presence of stanols as hydrogenated counterparts of sterols suggests that a process of hydrogenation is operating in soils.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2000-10-01 |