Carob leaf polyphenols trigger intrinsic apoptotic pathway and induce cell cycle arrest in colon cancer cells

6533b7d3fe1ef96bd1260a62

RESEARCH PRODUCT

Carob leaf polyphenols trigger intrinsic apoptotic pathway and induce cell cycle arrest in colon cancer cells

Dominique Delmas Babar Murtaza Danish Patoli Lionel Apetoh Mickaël Rialland François Ghringhelli Amira Sayed Khan Cédric Rébé Fatima Zahra Ghanemi Fatima Zahra Ghanemi Adélie Dumont Aziz Hichami Abdelhafid Nani Abdelhafid Nani Aurélie Fluckiger Charlotte De Rosny Ikram Aboura Ikram Aboura Meriem Belarbi Boucif Farid Lahfa Naim Akhtar Khan Chahid Benammar

subject

0301 basic medicine Cell cycle checkpoint animal diseases [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition Medicine (miscellaneous)Apoptosis Pharmacology digestive system Cell cycle arrest 03 medical and health sciences chemistry.chemical_compound Cytotoxic T cell TX341-641 Gallic acid IC50 Caspase Nutrition and Dietetics biology Nutrition. Foods and food supply Chemistry Carob (Ceratonia siliqua L.)food and beverages Polyphenols bacterial infections and mycoses Colorectal cancer 3. Good health stomatognathic diseases 030104 developmental biology Biochemistry Cell culture Apoptosis Polyphenol Caspases biology.protein [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition Food Science

description

IF 3.973; International audience; Chemoprevention of Colorectal cancer (CRC) is the major concern for improving public health. We investigated the protective effects of carob, Ceratonia siliqua L, leaf polyphenols (CLP) against CRC. Phenolic content analysis showed that CLP is enriched with gallic acid and m-coumaric acid. We observed that CLP exerted a dose dependent cytotoxic effect through the induction of apoptosis on CRC cell lines, with an IC50 around 20 mu g/mL CLP induced intrinsic apoptotic pathway through the caspase-9 activation and PARP cleavage in HCT-116 and CT-26 cells. Moreover, CLP induced cell cycle arrest in the G1 phase through p53 activation. Gallic acid and m-coumaric acid exerted similar effect of CLP but at higher concentration to that expected to be present in CLP, suggesting the synergistic effect between polyphenols in CLP activity. Interestingly, the carob leaf infusion reduced CT-26 tumour growth in BALB/c mice. This study suggests that CLP can be used for the prevention of CRC. (C) 2017 Elsevier Ltd. All rights reserved.

year	journal	country	edition	language
2017-06-01

https://hal-univ-bourgogne.archives-ouvertes.fr/hal-01536927