6533b7d3fe1ef96bd1260b84

RESEARCH PRODUCT

High-performance liquid chromatographic determination of tocopherols in infant formulas

Rosaura FarréReyes BarberáN RodrigoAmparo Alegría

subject

Detection limitChromatographyChemistryVitamine eOrganic ChemistryDetectorAnalytical chemistryInfantReproducibility of ResultsTocopherolsfood and beveragesLinearityGeneral MedicineSensitivity and SpecificityBiochemistryHigh-performance liquid chromatographyAnalytical ChemistrySpectrometry FluorescenceHumansInfant FoodQuantitative analysis (chemistry)Chromatography High Pressure LiquidAnalysis method

description

A method for the simultaneous determination of alpha-tocopherol acetate and alpha-, delta-, and gamma-tocopherols by normal-phase high-performance liquid chromatography (HPLC) with a fluorescent detector in infant formula is proposed. The values obtained in the determination of the analytical parameters: linearity, precision, limit of detection and accuracy (analysis of a standard reference material, SRM 1846), confirm the quality of the method. The proposed method is useful for the determination of alpha-, delta-, and gamma-tocopherols and alpha-tocopherol acetate in infant formulas at a low cost and in a total time of 2 h.

https://doi.org/10.1016/s0021-9673(01)01596-5