6533b7d3fe1ef96bd12613e3

RESEARCH PRODUCT

Application of integrative cloud point extraction and concentration for the analysis of polyphenols and alkaloids in mulberry leaves

Ping KouYu-jie FuZhi-ming LiuYi-wei JiangNa GuoThomas EfferthYan-yan Li

subject

Calibration curveLiquid-Liquid ExtractionClinical BiochemistryPharmaceutical Science01 natural sciencesHigh-performance liquid chromatographyAnalytical ChemistrySurface-Active AgentsRutinchemistry.chemical_compoundAlkaloidsChlorogenic acidDrug DiscoveryChromatography High Pressure LiquidSpectroscopyDetection limitCloud pointChromatography010405 organic chemistryChemistry010401 analytical chemistryExtraction (chemistry)Polyphenols0104 chemical sciencesPlant LeavesUltrasonic WavesPolyphenolSolventsMorus

description

Abstract A simple and efficient method based on cloud point extraction and concentration combined with high performance liquid chromatography was developed for the simultaneous separation and determination of five target compounds (deoxynojirimycin, chlorogenic acid, rutin, isoquercitrin and astragalin) in mulberry leaves samples. Firstly, to obtain a high extraction rate, the ultrasound assisted extraction was developed on acid modified Triton X-114 system. Under the optimal conditions, the total maximum extraction yields of five target compounds reached 20.80 mg/g, which was superior to conventional solvent extraction. After the cloud point extraction and concentration, the HPLC analysis parameters of calibration curve, intra-day and inter-day precision, limits of detection for the five target compounds were determined systematically. The proposed method was successfully used to extract, concentrate, separate and determine the polyphenols and alkaloids in mulberry leaves.

yearjournalcountryeditionlanguage
2018-11-12Journal of Pharmaceutical and Biomedical Analysis
https://doi.org/10.1016/j.jpba.2019.02.002