6533b7d4fe1ef96bd12620c7

RESEARCH PRODUCT

Modulation of Nitric Oxide Production by Tetracyclines and Chemically Modified Tetracyclines

Salvatore FeoViviana FerlazzoG. Di BellaManlio TolomeoEnrico CillariPietro D'agostinoM. La RosaCaterina BarberaGiuseppe CammarataSalvatore MilanoStefania Grimaudo

subject

Lipopolysaccharides0301 basic medicineLipopolysaccharideApoptosisNitric OxideDexamethasoneCell LineNitric oxideMice03 medical and health scienceschemistry.chemical_compound0302 clinical medicineWestern blotmedicineAnimalsInos proteinInhibitory effectomega-N-MethylarginineDose-Response Relationship Drugmedicine.diagnostic_testbiologyMacrophages030206 dentistryGeneral MedicineNitric oxide synthase030104 developmental biologychemistryBiochemistryTetracyclinesApoptosisInos mrnabiology.proteinNitric Oxide Synthase

description

Chemically modified tetracyclines (CMTs) dose-dependently decreased inducible nitric oxide synthase (iNOS) and, consequently, nitric oxide (NO) formation by the lipopolysaccharide (LPS)-stimulated J774 line. The inhibitory effect was due to a specific reduction in the iNOS protein content in the cells, as attested by Western blot analysis and by the inhibition of iNOS mRNA accumulation. Furthermore, CMTs cause a dose-dependent increase in cell death in the J774 line mediated by the NO-independent apoptotic mechanism.

yearjournalcountryeditionlanguage
1999-02-11Advances in Dental Research
https://doi.org/10.1177/08959374980120010701