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RESEARCH PRODUCT
Enzymatic, physicochemical, nutritional and phytochemical profile changes of apple (Golden Delicious L.) juice under supercritical carbon dioxide and long-term cold storage
ŁUkasz WoźniakJosé M. LorenzoKrystian MarszałekSylwia SkąpskaAlessandro ZambonSara SpilimbergoFrancisco J. Barbasubject
Time FactorsSucroseFood HandlingPhytochemicalsColorStorageCold storageAscorbic AcidPolyphenol oxidaseSubstrate SpecificityAnalytical Chemistrychemistry.chemical_compoundSupercritical carbon dioxide0404 agricultural biotechnologyPhenolsChlorogenic acidEnzyme activityVitamin CFood scienceCatechol oxidaseChromatography High Pressure LiquidColor; Enzyme activity; Polyphenols; Storage; Supercritical carbon dioxide; Synergistic effect; Vitamin C; Analytical Chemistry; Food ScienceVitamin CbiologyChemistryPolyphenolsfood and beverages04 agricultural and veterinary sciencesGeneral MedicineCarbon DioxideAscorbic acid040401 food scienceCold TemperatureFruit and Vegetable JuicesPeroxidasesPolyphenolMalusSynergistic effectbiology.proteinNutritive ValueCatechol OxidaseFood Sciencedescription
The impact of supercritical carbon dioxide (SCCD) (10-60 MPa/45 °C/30 min) and subsequent 10 weeks storage at 4 °C on polyphenol oxidase (PPO), peroxidase (POD) activities, phenolic profile, vitamin C, sugars, physicochemical properties of cloudy apple juices was investigated. No significant changes in sugars and total polyphenols were observed, whereas significant degradation (≈28%) of vitamin C and individual polyphenols (≈18%) was noted after SCCD treatment. After 4 weeks storage only 34% of vitamin C was retained and no vitamin C was detected after this time. Ten weeks of storage caused hydrolysis of sucrose in 15%, whereas degradation of individual polyphenols ranged from 43 to 50% depending on the pressure applied. The highest pressure was applied the highest retention of polyphenols was observed. The lightness of juice significantly increased by 15% after SCCD and decreased during storage. Moreover, the synergistic effect of both enzymes with chlorogenic acid and catechol was found.
year | journal | country | edition | language |
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2018-12-01 | Food Chemistry |