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RESEARCH PRODUCT
Assessment of microRNAs in patients with unstable angina pectoris.
Raphael TwerenboldEwa CzyzFrancisco OjedaChristian MuellerTobias ReichlinStefan BlankenbergMiriam ReiterTill KellerTanja ZellerThomas MünzelPhilipp S. WildStergios TzikasKarl J. Lacknersubject
OncologyAdultGenetic MarkersMalemedicine.medical_specialtyMyocardial InfarctionDiscriminatory powerInternal medicinemicroRNAmedicineHumansIn patientMyocardial infarctionAngina UnstableAgedUnstable anginabusiness.industryReproducibility of ResultsMiddle Agedmedicine.diseaseCirculating MicroRNAMicroRNAsEarly DiagnosisGenetic TechniquesROC CurveCase-Control StudiesCohortCardiologyBiomarker (medicine)FemaleCardiology and Cardiovascular Medicinebusinessdescription
Aims While cardiac troponin measurements have significantly improved the early diagnosis of myocardial infarction, the timely biomarker-based diagnosis of unstable angina pectoris (UAP) remains a major unmet clinical challenge. The aim of this study was to assess levels of circulating microRNAs (miRNAs) as possible novel biomarkers in patients with UAP. Methods and results A three-phase approach was conducted, comprising (i) profiling of miRNAs in patients with UAP and controls groups; (ii) replication of significant miRNAs in an independent patient cohort, (iii) validation of a multi-miRNAs panel in a third cohort. Out of 25 miRNAs selected for replication, 8 miRNAs remained significantly associated with UAP. In a validation phase, a miRNA panel including miR-132, miR-150, and miR-186 showed the highest discriminatory power [area under the receiver-operating-characteristic curve (AUC): 0.91; CI: 0.84–0.98]. Conclusion Using a profiling-replication-validation model, we identified eight miRNAs, which may facilitate the diagnosis of UAP.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-04-15 | European heart journal |