6533b7d5fe1ef96bd1263edb
RESEARCH PRODUCT
The role of accessory cells in polyclonal T cell activation. I. Both induction of interleukin 2 production and of interleukin 2 responsiveness by concanavalin A are accessory cell dependent
Michael LoosAnneliese SchimplThomas Hünigsubject
Malemusculoskeletal diseasesInterleukin 2medicine.medical_specialtyComplement Activating EnzymesT-LymphocytesT cellLymphocyte CooperationImmunologyPopulationchemical and pharmacologic phenomenaLymphocyte ActivationMiceInterleukin 21immune system diseasesInternal medicineConcanavalin AmedicineAnimalsImmunology and AllergyAntigen-presenting celleducationInterleukin 3LymphokinesMice Inbred BALB Ceducation.field_of_studybiologyComplement C1qImmune SeraHistocompatibility Antigens Class IIhemic and immune systemsCell biologyKineticsstomatognathic diseasesEndocrinologymedicine.anatomical_structurePolyclonal antibodiesConcanavalin Abiology.proteinInterleukin-2FemaleLymph NodesSpleenmedicine.drugdescription
Recent studies from other laboratories have shown that concanavalin A (Con A) acts at two separate steps in polyclonal T cell activation: interleukin 2 (IL2) production, and induction of responsiveness to IL2. Using a combination of techniques for the depletion of accessory cells from lymph node T cells, we have investigated which of these steps, if not both, is responsible for the known requirement for accessory cells in the Con A response. It was found that with increasing T cell purification, first the ability is lost to produce sufficient levels of endogenous IL2, whereas induction of IL2 responsiveness can still take place. Further removal of accessory cells however yields a population of resting T cells that cannot be induced by Con A to become IL2-reactive. It was concluded that both IL2 production and induction of reactivity to IL 2 are accessory cell-dependent events.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1983-01-01 | European Journal of Immunology |