6533b7d5fe1ef96bd12646ff

RESEARCH PRODUCT

3D reconstruction of the hemocyanin subunit dimer from the chiton Acanthochiton fascicularis.

J. Robin HarrisWolfgang GebauerJürgen MarklUlrich Meissner

subject

Dimermedicine.medical_treatmentProtein subunitPopulationGeneral Physics and AstronomyBiologylaw.inventionchemistry.chemical_compoundStructural BiologylawmedicineAnimalsGeneral Materials ScienceChitoneducationeducation.field_of_studyResolution (electron density)HemocyaninCell Biologybiology.organism_classificationNegative stainCrystallographyMicroscopy ElectronProtein SubunitschemistryMolluscaHemocyaninsElectron microscopeDimerization

description

Procedures are presented for the purification of the subunit dimer from Acanthochiton fasicularis hemocyanin. Electron microscopy of negatively stained specimens revealed a uniform population of macromolecules possessing the characteristic "boat shape". A 3D reconstruction from this EM data generated a approximately 3 nm resolution model that correlates well with earlier data of the purported subunit dimer, extracted from the 3D reconstruction of the didecamer of Haliotis tuberculata hemocyanin type 1.

yearjournalcountryeditionlanguage
2004-03-24Micron (Oxford, England : 1993)
10.1016/j.micron.2003.10.008https://pubmed.ncbi.nlm.nih.gov/15036283