6533b7d5fe1ef96bd126531b

RESEARCH PRODUCT

Automated Determination of Paroxetine and Its Main Metabolite by Column Switching and On-Line High-Performance Liquid Chromatography

A SzegediB HermesSebastian HärtterChristoph Hiemke

subject

ImipramineMetaboliteSensitivity and SpecificityHigh-performance liquid chromatographyImipramineMixed Function Oxygenaseschemistry.chemical_compoundCytochrome P-450 Enzyme SystemPiperidinesOral administrationDesipraminemedicineHumansDrug InteractionsPharmacology (medical)Chromatography High Pressure LiquidPharmacologyDetection limitChromatographyParoxetineParoxetineCytochrome P-450 CYP2D6chemistryFemaleQuantitative analysis (chemistry)medicine.drug

description

An automated column-switching method coupled to isocratic high-performance liquid chromatography has been developed for simultaneous determination of blood levels of paroxetine and its nonconjugated main metabolite BRL 36610. The lower limits of detection were 9-15 nmol/L (3-5 ng/ml) and linearity between drug concentration and detector response was found for 0-1,500 nmol/L (0-500 ng/ml). The method could be applied to the analysis of serum samples obtained from depressed patients who were treated with daily oral doses of 20 or 40 mg of paroxetine. After the 20-mg dose, the mean blood level of paroxetine was 69 nM (23 ng/ml), whereas the metabolite BRL 36610 was detectable in only one of 5 samples. Co-medication of paroxetine with imipramine increased the blood levels of imipramine, desipramine, and paroxetine thus indicating drug interactions.

yearjournalcountryeditionlanguage
1994-08-01Therapeutic Drug Monitoring
https://doi.org/10.1097/00007691-199408000-00011