6533b7d6fe1ef96bd1266623

RESEARCH PRODUCT

Hapten Synthesis and Polyclonal Antibody-Based Immunoassay Development for the Analysis of Forchlorfenuron in Kiwifruit

Celia Suárez-pantaleónAntonio Abad-somovillaAntonio Abad-fuentesConsuelo AgullóJosep V. Mercader

subject

ImmunoassayDetection limitAnalyteImmunogenChromatographymedicine.diagnostic_testbiologyPyridinesPhenylurea CompoundsActinidiaGeneral ChemistryForchlorfenuronAntibodieschemistry.chemical_compoundPlant Growth RegulatorschemistryLimit of DetectionPolyclonal antibodiesImmunoassaymedicinebiology.proteinGeneral Agricultural and Biological SciencesDerivatizationHaptensHapten

description

High-affinity polyclonal antibodies directed against the synthetic cytokinin forchlorfenuron (CPPU) were produced from three immunizing haptens with equivalent spacer arms located at different positions. A competitive immunoassay was developed with a limit of detection in buffer of 12.42 +/- 3.06 ng/L. In addition, the ability of the produced antibodies to recognize a set of synthetic CPPU analogues was studied. It was evidenced that the linker position had a strong impact on the specificity of the generated polyclonals, which were more sensitive to changes at moieties of the target analyte located furthest from the derivatization site of the immunogen. Finally, matrix effects of gold and green kiwifruit over assay parameters were evaluated. Excellent recoveries and low coefficients of variation were found with just a 100-fold dilution of the sample in buffer, hence indicating the effectiveness of the developed immunoassay as an analytical tool for monitoring this agrochemical in kiwifruit samples.

yearjournalcountryeditionlanguage
2010-08-05Journal of Agricultural and Food Chemistry
https://doi.org/10.1021/jf101660z