6533b7d6fe1ef96bd1266e25
RESEARCH PRODUCT
A comparison of various calibration techniques applied to the ninhydrin-cefoxitin determination
Pilar Campíns-falcóLuisa Gallo-martinezFrancisco Bosch-reigAdela Sevillano-cabezasubject
Chromatographymedicine.diagnostic_testChemistrydigestive oral and skin physiologyBiochemistryBlankHigh-performance liquid chromatographyAnalytical ChemistryAbsorbancechemistry.chemical_compoundSpectrophotometryNinhydrinReagentStandard additionmedicineEnvironmental ChemistryCefoxitinSpectroscopymedicine.drugdescription
Abstract This report deals with spectrophotometric determination of cefoxitin from intact antibiotics or from its acid induced degradation product by means of their reaction with ninhydrin in a strong sulphuric acid medium. To obtain the calibration graphs four methods were used: absorbance of reacted cefoxitin against a reagent blank as an analytical signal, first-derivative signal of absorbance against a water blank as an analytical signal, first-derivative signal of absorbance against a reagent blank as an analytical signal and the H-Point Standard Additions Method (HPSAM) by using absorbance increment values as analytical signals. The HPSAM is applied to the determination of cefoxitin in the presence of its acid-degraded product. Ninhydrin-cefoxitin determination is compared with reversed-phase HPLC determination and with the iodometric method.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1996-11-01 | Analytica Chimica Acta |