6533b7d6fe1ef96bd1266ebb

RESEARCH PRODUCT

Fluorogenic Tetrazine-Siliconrhodamine Probe for the Labeling of Noncanonical Amino Acid Tagged Proteins

Eszter KozmaGemma Estrada GironaEdward A. LemkeEdward A. LemkePéter KeleGiulia Paci

subject

0301 basic medicinechemistry.chemical_classificationSuper-resolution microscopy010402 general chemistry01 natural sciencesFluorescence0104 chemical sciencesAmino acid03 medical and health sciencesTetrazinechemistry.chemical_compoundFluorescent labelling030104 developmental biologychemistryBiophysics

description

Tetrazine-bearing fluorescent labels enable site-specific tagging of proteins that are genetically manipulated with dienophile modified noncanonical amino acids. The inverse electron demand Diels-Alder reaction between the tetrazine and the dienophile fulfills the criteria of bioorthogonality allowing fluorescent labeling schemes of live cells. Here, we describe the detailed synthetic and labeling protocols of a near infrared emitting siliconrhodamine-tetrazine probe suitable for super-resolution imaging of residue-specifically engineered proteins in mammalian cells.

yearjournalcountryeditionlanguage
2018-01-01
https://doi.org/10.1007/978-1-4939-7574-7_22