6533b7d7fe1ef96bd1267883
RESEARCH PRODUCT
Über den Abbau von L-Äpfelsäure durch Hefen verschiedener Gattungen mit Malatenzym
F. RadlerE. Fucksubject
chemistry.chemical_classificationbiologyfungiMalic enzymefood and beveragesGeneral MedicineZygosaccharomycesbiology.organism_classificationMalate dehydrogenaseYeastchemistry.chemical_compoundchemistryBiochemistryOxidoreductaseFermentationMalic acidPichiadescription
Summary (1) The aerobic assimilation of malic acid is not a character of certain yeast genera or species as was shown by testing more than 300 different strains. Single strains of the following-species were found to grow on malic acid as the only carbon source: Candida pulcherrima, C. utilis, C. mycoderma, Torulopsis famata, Pichia membranaefaciens, P. wickerhamii, Hansenula capsulata, Trigonopsis variabilis , and Zygosaccharomyces chevalieri . (2) During fermentation C. pulcherrima and T. famata decompose up to 40% and C. utilis up to 80% of the L-malic acid that is present in the medium. (3) L-Malic acid is decomposed to CO 2 and the corresponding amounts of ethanol or pyruvate by cell free extracts of yeasts of the genera Pichia, IIansenula, Trigonopsis, Zygosaccharomyces, Kloeckera, Candida , and Torulopsis . (4) The enzymatic activity of malate dehydrogenase (L-malate: NAD oxidoreductase, E.C.1.1.1.37) was separated from the decarboxylating enzyme by gel chromatography of extracts of some strains of the genera Pichia, Candida , and Hansenula . It is assumed that malic acid is decomposed by a malic enzyme (L-malate: NAD oxidoreductase, decarboxylating E.C. 1.1.1.38) that was partially purified and found to have a molecular weight of 120000–130000.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1974-01-01 | Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Zweite Naturwissenschaftliche Abteilung: Allgemeine, Landwirtschaftliche und Technische Mikrobiologie |