Phytochemical profile and bioactivity of traditional ayurvedic decoctions and hydro-alcoholic macerations of Boerhaavia diffusa L. and Curculigo orchioides Gaertn.

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RESEARCH PRODUCT

Phytochemical profile and bioactivity of traditional ayurvedic decoctions and hydro-alcoholic macerations of Boerhaavia diffusa L. and Curculigo orchioides Gaertn.

Gianni Sacchetti Alessandra Guerrini Renato Bruni Massimo Tacchini Matteo Marieschi Augusta Caligiani Antonella Spagnoletti Thomas Efferth

subject

DPPH Phytochemicals antioxidant activity Decoction Plant Science Biochemistry Plant Roots Antioxidants Analytical Chemistry NO chemical fingerprinting Ferulic acid chemistry.chemical_compound Curculigo Phenols Eupalitin Cell Line Tumor Organic chemistry Humans Curculigoside Boerhaavia diffusa; Curculigo orchioides; traditional preparations; chemical fingerprinting; antioxidant activity; genotoxicity; cytotoxicity traditional preparations biology Traditional medicine Plant Extracts Curculigo orchioides Organic Chemistry genotoxicity biology.organism_classification Antineoplastic Agents Phytogenic Curculigo orchioides Medicine Ayurvedic Hypoxidaceae Boerhaavia diffusa chemistry cytotoxicity Chemical fingerprinting Nyctaginaceae

description

Decoctions (DECs) and hydro-alcoholic extracts (HEs) prepared from roots of Boerhaavia diffusa L. (Nyctaginaceae) and Curculigo orchioides Gaertn. (Hypoxidaceae) were phytochemically characterised by HPLC-DAD and profiled for their antioxidant, antigenotoxic and cytotoxic activities. B. diffusa DEC was rich in ferulic acid and vanillin, while the HE also contained boeravinone B and eupalitin. Both C. orchioides HE and DEC displayed the main occurrence of orcinol-β-d-glucoside and curculigoside A. Antioxidant activity was assayed through spectrophotometric DPPH, ABTS and β-carotene bleaching test, and using (HP)TLC bioautographic strategies. For both crude drugs, HE was the best performing preparation. Properly modified SOS-Chromotest evidenced a 10% inhibition by phytocomplexes against 4-nitroquinoline-N-oxide, and a higher bioactivity for vanillin (36.60 ± 1.68%) and ferulic acid (35.09 ± 1.53%). C. orchioides HE was the preparation which showed higher cytotoxicity against drug-sensitive human T-lymphoblastoid cell line (CCRF-CEM) and multidrug-resistant leukaemia cell line (CEM/ADR5000), and eupalitin was the only pure compound to exhibit an IC50 value.

year	journal	country	edition	language
2015-01-01

10.1080/14786419.2014.1003299 http://hdl.handle.net/11392/2294419