6533b7d7fe1ef96bd1268541

RESEARCH PRODUCT

Extracellular space and electrolyte distribution in cortex and white matter of dog brain in cold induced oedema

subject

description

24 hours after a circumscribed cold injury of the cortex dog brains were perfused from the lateral ventricle and the frontal subarachnoidal space to the cisterna magna with an artificial CSF containing trace amounts of35S-labelled thiosulphate. Simultaneously the extracellular tracer was administered intravenously. Extracellular fluid volume was estimated and found to be increased from 10 to 15% in the oedematous cortex and from 10 to 27% in the oedematous white matter. The actual size of ECS in oedematous white matter, however, must be larger as indicated by the relative alterations of thiosulphate distribution, tissue water, sodium and chloride. Apparently a small part of the fluid accumulation affects the cellular compartment in oedematous white matter. It may be concluded from the close spatial correspondence of the spreading of I131 albumin and Evans blue, the increase in water and sodium content, and the enlargement of the TSS that the dilated extracellular channels are filled with a plasma like oedema fluid, derived from blood.