6533b7d8fe1ef96bd126ab96

RESEARCH PRODUCT

Flow Cytometric Analysis of the Expression Pattern of Peroxisomal Proteins, Abcd1, Abcd2, and Abcd3 in BV-2 Murine Microglial Cells

Amira ZarroukAmira ZarroukSébastien TerreauImen HelaliImen HelaliFlore GeillonMaryem BezineMaryem BezineThomas NuryAmina NajidEl Mostafa KarymAnne VejuxMustapha Cherkaoui-malkiPierre AndreolettiMeryam DebbabiMeryam DebbabiDoriane TrompierCatherine GondcailleGérard LizardStéphane Savary

subject

0301 basic medicinemedicine.diagnostic_testChemistryContext (language use)PeroxisomeMolecular biologyFlow cytometryCell biology03 medical and health sciences030104 developmental biologyCytoplasmCell cultureGene expressionFluorescence microscopemedicineImmortalised cell line

description

Microglial cells play important roles in neurodegenerative diseases including peroxisomal leukodystrophies. The BV-2 murine immortalized cells are widely used in the context of neurodegenerative researches. It is therefore important to establish the expression pattern of peroxisomal proteins by flow cytometry in these cells. So, the expression pattern of various peroxisomal transporters (Abcd1, Abcd2, Abcd3) contributing to peroxisomal β-oxidation was evaluated on BV-2 cells by flow cytometry and complementary methods (fluorescence microscopy, and RT-qPCR). By flow cytometry a strong expression of peroxisomal proteins (Abcd1, Abcd2, Abcd3) was observed. These data were in agreement with those obtained by fluorescence microscopy (presence of numerous fluorescent dots in the cytoplasm characteristic of a peroxisomal staining pattern) and RT-qPCR (high levels of Abcd1, Abcd2, and Abcd3 mRNAs). Thus, the peroxisomal proteins (Abcd1, Abcd2, Abcd3) are expressed in BV-2 cells, and can be analyzed by flow cytometry.

yearjournalcountryeditionlanguage
2017-01-01
https://doi.org/10.1007/978-1-4939-6937-1_25