6533b7d8fe1ef96bd126ad77

RESEARCH PRODUCT

Transmurality of scar influences the effect of a hybrid-intervention with autologous bone marrow cell injection and aortocoronary bypass surgery (MNC/CABG) in patients after myocardial infarction

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Abstract Background Cell therapy (CTx) is a strategy to support cardiac regeneration after myocardial infarction (MI). Thus far, clinical studies provided mixed results. Here, we investigated whether transmurality of the infarct may play a relevant role. Methods 18 patients (63±3years, 15 male) undergoing elective coronary artery bypass graft (CABG) surgery 2.2±0.7months post MI participated. 10 had transmural and 8 non-transmural infarct scars assessed by Tc-99m-MIBI Single-Photon Emission Computed Tomography (SPECT) and F18-FDG-Positron-Emission-Tomography (PET). During surgery, 10ml of sternal bone marrow were obtained, mononuclear cells (MNC) were isolated. At the end of surgery MNC were injected into the infarctions' center and border zones (10 injections, 2ml total, 6.6±1.3×10 7 MNC). Results No major complications attributable to cell therapy were observed. The sizes of non-transmural scars were reduced at 3 and 24months after treatment (7.7±1.1% and 5.5±1.8 vs. 17.5±4.9%, P=0.05 and P=0.04), while transmural scars remained unchanged (23.5±2.6% and 23.8±3.2 vs. 23.5±2.6%, P>0.99 and P=0.95). A trend towards improved LVEF was seen in patients with non-transmural scars (MRI: 48.8±5.1% vs. 30.6±8.7%, P=0.3; SPECT: 54.1±3.1 vs. 41.0±4.0, P=0.086), but not in patients with transmural scars (MRI: 36.7±3.9 vs. 34.3±5.0, P=0.63, SPECT: 37.8±3.1 vs. 37.9±2.3%, P=0.96). Conclusions A single hybrid intervention of MNC recovery, purification and injection with CABG-surgery (MNC/CABG) may be an attractive modality for cell therapy. However, no regeneration of avital transmural scar tissue seems to occur, while the contribution of MNC to improved perfusion in non-transmural myocardial infarct scars remains to be determined.