Hepatīta C vīrusa ARFP ekspresija Escherichia coli un īpašību raksturošana

6533b7d8fe1ef96bd126b179

RESEARCH PRODUCT

Hepatīta C vīrusa ARFP ekspresija Escherichia coli un īpašību raksturošana

Santa Andersone

subject

ekspresija ARFP HCV Bioloģija IMAC

description

Ar C hepatīta vīrusu inficēto cilvēku skaits pasaulē ir apmēram 170 miljoni. Pret HCV nav vakcīnas, tāpēc tiek veikti pētījumi, kas saistīti ar tās izstrādi. Darba mērķis bija iegūt HCV alternatīvā nolasīšanas rāmja proteīnus divās plazmīdu sistēmās, un optimizēt ekspresijas un attīrīšanas apstākļus augstas kvalitātes proteīnu iegūšanai. Darbā tika izmantoti dažāda garuma un genotipa ARFP un divas klonēšanas un ekspresijas plazmīdas ar atšķirīgu 6xHistag iezīmes novietojumu. Pēc indukcijas ar IPTG apstākļu optimizācijas augstākā ARFP ekspresija E. coli BL21(DE3) šūnās tika iegūta, inducējot 4h pie + 37°C un 6h pie + 30°C ar 0,4 mM IPTG. Pēc ARFP attīrīšanas ar imobilizēto metālafīno hromatogrāfiju tika konstatēts, ka proteīni ir daļēji šķīstoši un nedegradē, tāpēc tos ir plānots izmantot imunoloģiskiem pētījumiem dzīvnieku modelī.

year	journal	country	edition	language
2017-01-01

https://dspace.lu.lv/dspace/handle/7/36201

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