6533b7d8fe1ef96bd126b6f0
RESEARCH PRODUCT
In situ analysis of the bacterial communities associated to farmed eel by whole-cell hybridization.
Rosa AznarRosa AznarYolanda MorenoH. MeierCovadonga R. AriasEsperanza Garaysubject
endocrine systemanimal structuresColony Count MicrobialVibrio vulnificusAquacultureApplied Microbiology and BiotechnologyMicrobiologyVibrionaceae23S ribosomal RNARNA Ribosomal 16SmedicineAnimalsIn Situ Hybridization FluorescencePhylogenyAlphaproteobacteriaVibrioEelsbiologymedicine.diagnostic_testBacteriafungiBetaproteobacteriaequipment and suppliesbiology.organism_classificationVibrioRNA BacterialRNA Ribosomal 23SbacteriaProteobacteriaMolecular probeOligonucleotide ProbesWater MicrobiologyBacteriaFluorescence in situ hybridizationdescription
Bacterial communities in water samples and eel slime were investigated by fluorescence in situ hybridization of whole bacterial cells in an eel intensive culture system over 1 year. A newly developed probe, matching 27 Vibrio spp., and a specific probe for Vibrio vulnificus were used. Phylogenetic probes complementary to selected regions of the 16S and 23S ribosomal RNA revealed that Proteobacteria of the alpha and beta subclass were predominant in water and eel slime. Members of the gamma subclass (e.g. vibrios and aeromonads) were more abundant in eel slime, although no V. vulnificus was detected.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1999-10-26 | Letters in applied microbiology |