6533b7d9fe1ef96bd126ba48
RESEARCH PRODUCT
Quantitative contributions of IgG, IgM and C3 to erythrophagocytosis and rosette formation by peritoneal macrophages, and anti-opsonin activity of dextran sulfate 500.
Brigitte WellekH HahnW. Opferkuchsubject
ErythrocytesPhagocytosisImmunologyGuinea PigsDose-Response Relationship ImmunologicHemolysisMicrobiologyGuinea pigImmune systemPhagocytosismedicineCell AdhesionImmunology and AllergyAnimalsIncubationSensitizationbiologyMacrophagesImmune adherenceDextransComplement C3Complement System ProteinsOpsonin ProteinsIn vitroImmune Adherence Reactionmedicine.anatomical_structureImmunoglobulin MImmunoglobulin Gbiology.proteinAntibodydescription
In vitro phagocytosis by guinea pig peritoneal macrophages of immune complexes (EA) was shown to be dependent on IgG antibody in a dose-dependent fashion. C3b enhanced phagocytosis of EA at limited IgG antibody concentrations only. When IgM antibody was used for sensitization of sheep red blood cells (SRBC), phagocytosis and rosette formation did not occur in the absence of bound C3. The polyanion, dextran sulfate 500 (DS), was shown to depress both rosette formation and phagocytosis of EAIgG, C1423 and EAIgMC1423, as well as immune adherence of human group 0 erythrocytes and hemolytic activity of C3. This effect of DS was seen only when it was actually present in the incubation medium.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1976-06-01 | European journal of immunology |