6533b7d9fe1ef96bd126c17a
RESEARCH PRODUCT
Anhydrobiosis in yeasts: Psychrotolerant yeasts are highly resistant to dehydration.
Galina KhroustalyovaBenedetta TurchettiGaia GiovannittiDaria SeveriniPietro BuzziniAlexander RapoportRita Scherbakasubject
0106 biological sciencesBioengineeringBiology01 natural sciencesApplied Microbiology and BiotechnologyBiochemistryPermeabilityCell membrane03 medical and health sciences010608 biotechnologyYeastsGeneticsmedicinedehydration–rehydrationViability assayDesiccationCryptobiosis030304 developmental biology0303 health sciencesMicrobial ViabilityMicrobial ViabilityDehydrationCell MembraneMetabolismanhydrobiosisYeastanhydrobiosis; dehydration–rehydration; plasma membrane permeability; resistance to desiccation; Cell Membrane; Desiccation; Permeability; Yeasts; Dehydration; Microbial ViabilityMembranemedicine.anatomical_structureBiochemistryDesiccationplasma membrane permeabilityresistance to desiccationBiotechnologydescription
Yeast cells are able to transition into a state of anhydrobiosis (temporary reversible suspension of metabolism) under conditions of desiccation. One of the most efficient approaches for understanding the mechanisms underlying resistance to dehydration-rehydration is to identify yeasts, which are stable under such treatments, and compare them with moderately resistant species and strains. In the current study, we investigated the resistance to dehydration-rehydration of six psychrotolerant yeast strains belonging to two species. All studied strains of Solicoccozyma terricola and Naganishia albida were found to be highly resistant to dehydration-rehydration. The viability of S. terricola strains was close to 100%. Such results have not been previously reported in studies of anhydrobiosis in yeasts. The plasma membrane changes, revealed by determining its permeability under various rehydration conditions, were also surprisingly minimal. Thus, the high level of resistance of psychrotolerant yeast strains might be related to the chemical composition and molecular organisation of their plasma membranes. Aside from plasma membrane characteristics, other important factors may also influence the maintenance of yeast cell viability under conditions of dehydration-rehydration.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-10-10 | Yeast (Chichester, England) |