6533b7d9fe1ef96bd126cd34
RESEARCH PRODUCT
NGF and TGF-beta mRNA expression during pregnancy in a rat corneal wound healing model.
A NietoF Martinez-sorianoJ CasasLuis Garcia AlonsoS M RahhalJuan A. Sanchis-gimenosubject
Gene isoformPathologymedicine.medical_specialtyMrna expressionAndrologyCorneaRats Sprague-DawleyPregnancyTransforming Growth Factor betaCorneaNerve Growth FactormedicineAnimalsRNA MessengerMessenger RNAPregnancyWound Healingbusiness.industrymedicine.diseaseeye diseasesRatsOphthalmologymedicine.anatomical_structureCorneal woundPregnancy AnimalFemalesense organsWound healingbusinessOptometryTransforming growth factorCorneal Injuriesdescription
Background Growth factors seem to play a major role in corneal wound healing and TGF-beta seems to be associated with abnormal healing after corneal surgical procedures. Few studies have analysed the role of NGF and TGF-beta on corneal wound healing during pregnancy. The aim of the present study was to create an animal model to evaluate the expression of NGF and TGF-betas during corneal wound healing in two groups: control and pregnant rats. Methods Corneal mRNA for NGF and the three isoforms of TGF-beta were analysed by RT-PCR, in a time-course experiment on different days after epithelial wounding (2, 7, 14 days) in pregnant and control groups Results The results show high corneal mRNA expression for NGF and TGF-beta1 without any variation throughout the healing process or pregnancy evolution. However, we detected a different expression of corneal mRNAs for TGF-beta2 and TGF-beta3 in the control group. This data was not detected in the pregnant group. Discussion Our results suggested that pregnancy could have a relevant role on TGF-beta2 and TGF-beta3 mRNA expression during the corneal wound healing process. Additional research should be performed to corroborate these findings.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2003-07-01 | Clinicalexperimental optometry |