6533b7d9fe1ef96bd126cef8
RESEARCH PRODUCT
Cytotoxicity of Root Canal Filling Materials to Three Different Human Cell Lines
R SchulzeDirk SchäferBrita WillershausenBenjamín Briseño Marroquínsubject
Materials scienceCell SurvivalRoot canalmedicine.medical_treatmentStatistics as TopicCellGingivaDentistryBiocompatible MaterialsCell morphologyDinoprostoneCell LineCalcium HydroxideRoot Canal Filling Materialschemistry.chemical_compoundTumor Cells CulturedmedicineHumansNeoplasms Glandular and EpithelialViability assayCytotoxicityGeneral DentistryCalcium hydroxideEpoxy ResinsCell growthbusiness.industryProteinsFibroblastsMolecular biologySalicylatesNasal Mucosamedicine.anatomical_structurechemistryGlass Ionomer CementsDentin-Bonding AgentsGutta-PerchabusinessBiomarkersCell DivisionProstaglandin Edescription
The aim of this study was to investigate the biological compatibility of five root canal sealers (Sealapex, Endion, Super-EBA, Ketac-Endo, and AH Plus) and regular and calcium hydroxide-based gutta-percha in three different human cell lines. Cultures without root canal sealers were used as controls. Cell growth, cell morphology, cell viability, protein content of the cells, and prostaglandin E 2 (PGE 2 ) release were used as parameters to determine the cytotoxicity of the materials. The protein content of the three cell lines—nasal fibroblasts, gingival fibroblasts, and epithelial tumor cells—was significantly reduced (p ≤ 0.001) by all materials tested. Determinations of PGE 2 release showed significant material specific differences. No significant increased PGE 2 release values were found with Sealapex, Endion, and Super-EBA. On the contrary significantly increased PGE 2 levels were measured with Ketac Endo, AH Plus, regular and calcium hydroxide-based gutta-percha points (p ≤ 0.001).
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2001-07-27 | Journal of Endodontics |