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RESEARCH PRODUCT
Preparation of Anti-protein and Anti-mannan Antisera against Fungal Cell Wall by Affinity Chromatography
Maria ÀNgels Cebrià I IranzoRafael SentandreuSalvador MormeneoAntonio MarcillaM V Elorzasubject
Antiserumeducation.field_of_studymedicine.diagnostic_testPopulationYarrowiaBiologyImmunofluorescencebiology.organism_classificationApplied Microbiology and Biotechnologycarbohydrates (lipids)Affinity chromatographyBiochemistryPolyclonal antibodiesmedicinebiology.proteinCandida albicanseducationMannandescription
Abstract Iranzo, M., Marcilla, A., Elorza, M. V., Mormeneo, S., and Sentandreu, R. 1994. Preparation of anti-protein and anti-mannan antisera against fungal cell wall by affinity chromatography. Experimental Mycology 18, 159-167. A novel and easy chromatographic method has been developed for the isolation of anti-protein and anti-mannan antisera from a population of polyclonal antibodies obtained against Candida albicans and Yarrowia lipolytica cell wall mannoproteins. The technique is based on the immobilization of mannan (to be used as immunoadsorbent) by Affi-Prep H z resin after the oxidation of neighboring hydroxyl groups of the polysaccharide with sodium periodate. For Y. lipolytica polyclonal antiserum, a single chromatographic step using the homologous mannan was sufficient to obtain an antiprotein antibody preparation free of antimannan antibodies. For C. albicans , three chromatographic processes using homologous and heterologous mannan were needed to obtain a satisfactory antiprotein antiserum. The potential application of the anti-protein antiserum obtained has been demonstrated by indirect immunofluorescence assays of whole cells and electrophoretic analysis of wall proteins in C. albicans and Saccharomyces cerevisiae .
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1994-06-01 | Experimental Mycology |