6533b7dafe1ef96bd126e33b
RESEARCH PRODUCT
Dual inhibition of cyclooxygenase-1 and 5-lipoxygenase by aerial part of Bupleurum fruticescens methanol extract
José-luis RíosJose M. PrietoRosa-maría GinerSalvador MáñezMaría-carmen Reciosubject
BupleurumLipoxygenaseFlowersPlant RootsInhibitory Concentration 50LipoxygenasePhospholipase A2Drug DiscoveryAnimalsHumansCyclooxygenase InhibitorsRats WistarPharmacologychemistry.chemical_classificationChromatographybiologyPlant ExtractsElastaseMembrane ProteinsGeneral MedicinePlant Components Aerialbiology.organism_classificationBupleurumRatsIsoenzymesEnzymeEicosanoidchemistryBiochemistryProstaglandin-Endoperoxide SynthasesArachidonate 5-lipoxygenaseCyclooxygenase 1biology.proteinlipids (amino acids peptides and proteins)CyclooxygenaseLeukocyte ElastasePhytotherapydescription
The effect of the methanol extract from aerial parts of Bupleurum fruticescens on the release of eicosanoids and hydrolytic enzymes was determined on in vitro cell systems. The extract had a significant effect on 5-lipoxygenase (5-LOX) activity, inhibiting both LTB4 and 5(S)-HETE production with IC50 values of 112 microg/ml and 95 microg/ml, respectively. At concentrations of 200 microg/ml, the extract also inhibited cyclooxygenase-1 (90%) and elastase activities (54%). The 12-LOX activity in intact platelets was not affected; a fact, which suggests that phospholipase A2 (PLA2) activity, is not modified by the extract.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2003-10-03 | Fitoterapia |