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RESEARCH PRODUCT

Morphogenetically-Active Barrier Membrane for Guided Bone Regeneration, Based on Amorphous Polyphosphate

Maximilian AckermannShunfeng WangMeik NeufurthXiaohong WangWerner E. G. MüllerHeinz C. Schröder

subject

0301 basic medicineBone Regenerationcollagen-inducingBarrier membranePolymersPharmaceutical Science02 engineering and technologyMatrix (biology)chemistry.chemical_compoundMiceOsteogenesisPolyphosphatesDrug Discoverystromal cell-derived factor-1Pharmacology Toxicology and Pharmaceutics (miscellaneous)MC3T3-E1 cellsChemistrybiologizationAnatomy3T3 Cells021001 nanoscience & nanotechnology3. Good healthMembranetensile strength/resistanceAlkaline phosphataseCollagen0210 nano-technologyinorganic polyphosphateSurface PropertiesPolyestersArticleAngiopoietin-203 medical and health sciencesCalcification PhysiologicAnimalsHumansBone regenerationTissue EngineeringPolyphosphateMesenchymal stem cellMembrane ProteinsMembranes ArtificialMesenchymal Stem Cellspolypropylene mesh030104 developmental biologyGene Expression RegulationBiophysicsbiologization; hernia repair; inorganic polyphosphate; collagen-inducing; polypropylene mesh; tensile strength/resistance; stromal cell-derived factor-1; MC3T3-E1 cellsNanoparticlesWound healinghernia repair

description

We describe a novel regeneratively-active barrier membrane which consists of a durable electrospun poly(ε-caprolactone) (PCL) net covered with a morphogenetically-active biohybrid material composed of collagen and inorganic polyphosphate (polyP). The patch-like fibrous collagen structures are decorated with small amorphous polyP nanoparticles (50 nm) formed by precipitation of this energy-rich and enzyme-degradable (alkaline phosphatase) polymer in the presence of calcium ions. The fabricated PCL-polyP/collagen hybrid mats are characterized by advantageous biomechanical properties, such as enhanced flexibility and stretchability with almost unaltered tensile strength of the PCL net. The polyP/collagen material promotes the attachment and increases the viability/metabolic activity of human mesenchymal stem cells compared to cells grown on non-coated mats. The gene expression studies revealed that cells, growing onto polyP/collagen coated mats show a significantly (two-fold) higher upregulation of the steady-state-expression of the angiopoietin-2 gene used as an early marker for wound healing than cells cultivated onto non-coated mats. Based on our results we propose that amorphous polyP, stabilized onto a collagen matrix, might be a promising component of functionally-active barrier membranes for guided tissue regeneration in medicine and dentistry.

yearjournalcountryeditionlanguage
2017-05-01Marine Drugs
10.3390/md15050142http://europepmc.org/articles/PMC5450548