6533b7dafe1ef96bd126f70c
RESEARCH PRODUCT
A real time immunoassay in alumina membranes
Marina CretichTormod VoldenMarcella ChiariK. B. GylfassonLaura SolaMarcus J. SwannJesús ÁLvarezDaniel Hillsubject
Other Electrical Engineering Electronic Engineering Information EngineeringMaterials sciencemedicine.diagnostic_testbusiness.industryAlumina membranesNanotechnologyMembraneporous aluminaQuantum dotPorous membraneImmunoassayCopolymermedicineAnnan elektroteknik och elektronikPhotonicsbusinessBiosensorBiosensordescription
To date, photonic biosensing with porous membranes has produced slow responses and long sensing times, due to the narrow (less than 100 nm) closed end pores of the membranes used. Recently, polarimetry was used to demonstrate analyte flow through, and real time biosensing in, free-standing porous alumina membranes. Here, we demonstrate how an improved functionalization technology, has for the first time enabled a real-time immunoassay within a porous membrane with a total assay time below one hour. With the new approach, we show a noise floor for individual biosensing measurements of 3.7 ng/ml (25 pM), and a bulk refractive index detection limit of 5×10-6 RIU, with a standard deviation of less than 5%. The membranes, with their 200 nm pore diameter enabling targeted delivering of analytes to bioreceptors immobilized on the pore walls, therefore provide a route towards rapid and low cost real-time opto-fluidic biosensors for small sample volumes. QC 20150203 Positive
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-11-01 | IEEE SENSORS 2014 Proceedings |