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RESEARCH PRODUCT

Ultraviolet B Irradiation Modulates the Immune System of Fish (Rutilus rutilus, Cyprinidae) Part III: Lymphocytes¶

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Abstract The effects of short-term exposure to ultraviolet B (UVB) radiation on lymphocyte-related parameters were studied under controlled laboratory conditions using roach (Rutilus rutilus), a cyprinid teleost, as the model fish. In vitro lymphoproliferative responses stimulated with a T-cell–specific mitogen, concanavalin A (ConA), or a B-cell–specific activator, lipopolysaccharide (LPS), were decreased in exposed fish. Also nonstimulated proliferation was lower than in unexposed fish. ConA-activated responses returned to normal levels within 7 days after exposure, but LPS-activated responses were reduced throughout the 14 day follow-up. The capability of UVB-exposed fish to produce an antibody response was studied by intraperitoneal immunization with bovine γ-globulin (BGG). The concentration of anti-BGG antibodies in plasma as well as the number of anti-BGG–specific antibody-secreting cells in the spleen or blood were not decreased in fish exposed either to a single dose of UVB prior to immunization,...