0000000000293774
AUTHOR
T.m. Aaltonen
showing 10 related works from this author
Immunoreactivity of Roach,Rutilus rutilus,Following Laboratory Exposure to Bleached Pulp and Paper Mill Effluents
1998
Abstract In order to study immunomodulation, controlled laboratory experiments were carried out with roach ( Rutilus rutilus ) exposed to bleached kraft mill effluent (BKME) or spent bleach liquor (SBL) from two pulp and paper mills using elementary chlorine and chlorine dioxide for bleaching. The total number of immunoglobulin-secreting cells (ISC) and the number of specific antibody-secreting cells (ASC) were determined by ELISPOT assay. Exposure to BKME resulted in decreased numbers of ISC in the spleen. To study the capability of response against foreign antigens the fish were immunized with bovine γ-globulin. Exposure to BKME or SBL reduced antigen-specific ASC response in fish. Moreov…
Comparative effects of UVA and UVB irradiation on the immune system of fish.
2000
Aquatic organisms can be harmed by the current levels of solar ultraviolet radiation. We have recently shown that exposure of fish to UVB irradiation alters the functioning of the fish immune system, but the effects of UVA radiation are unknown. The present study continues this work by characterizing UVA irradiation-induced immunological changes in fish. Roach, a cyprinid fish, were exposed to a single dose of either UVA (3.6 J/cm2) or UVB (0.5 J/cm2) irradiation. Both irradiations suppressed transiently mitogen-stimulated proliferation of blood lymphocytes. UVA, but not UVB, decreased hematocrit, plasma protein, and plasma immunoglobulin levels and increased the proportions of blood cells …
Ultraviolet B Irradiation Modulates the Immune System of Fish (Rutilus rutilus, Cyprinidae) Part III: Lymphocytes¶
2001
Abstract The effects of short-term exposure to ultraviolet B (UVB) radiation on lymphocyte-related parameters were studied under controlled laboratory conditions using roach (Rutilus rutilus), a cyprinid teleost, as the model fish. In vitro lymphoproliferative responses stimulated with a T-cell–specific mitogen, concanavalin A (ConA), or a B-cell–specific activator, lipopolysaccharide (LPS), were decreased in exposed fish. Also nonstimulated proliferation was lower than in unexposed fish. ConA-activated responses returned to normal levels within 7 days after exposure, but LPS-activated responses were reduced throughout the 14 day follow-up. The capability of UVB-exposed fish to produce an a…
Exploitation of Microtubule Cytoskeleton and Dynein during Parvoviral Traffic toward the Nucleus
2003
ABSTRACT Canine parvovirus (CPV), a model virus for the study of parvoviral entry, enters host cells by receptor-mediated endocytosis, escapes from endosomal vesicles to the cytosol, and then replicates in the nucleus. We examined the role of the microtubule (MT)-mediated cytoplasmic trafficking of viral particles toward the nucleus. Immunofluorescence and immunoelectron microscopy showed that capsids were transported through the cytoplasm into the nucleus after cytoplasmic microinjection but that in the presence of MT-depolymerizing agents, viral capsids were unable to reach the nucleus. The nuclear accumulation of capsids was also reduced by microinjection of an anti-dynein antibody. More…
Antibody synthesis in roach (Rutilus rutilus); analysis of antibody secreting cells in lymphoid organs with ELISPOT-assay
1994
The roach (Rutilus rutilus L.) which is a cyprinid fish, was immunised with bovine γ-globulin (BGG) and the antibody synthesis was studied by counting the number of specific antibody secreting cells (SASC) in the spleen and anterior kidney, and by measuring the antibody concentration in the circulation. SASCs and the total number of immunoglobulin secreting cells (TISC) were counted with the ELISPOT (enzyme-linked immunospot) assay, and anti-BGG antibodies and the concentration of immunoglobulin in sera were assayed by ELISA (enzyme-linked immunosorbent assay). The present modification of the ELISPOT-assay takes advantage of biotin-avidin amplification and yields easily detectable and nonfa…
Humoral response of roach (Rutilus rutilus) to digenean Rhipidocotyle fennica infection
1997
The humoral immune response of roach (Rutilus rutilus) to cercariae of the digenean trematode, Rhipidocotyle fennica, was studied. Antibodies against R. fennica were found in wild roach in lakes where fish are infected by the parasite. Antibody levels were higher in sera collected in September than in sera collected in June, due to infection of R. fennica during the late summer. In experimental aquarium studies, roach immunized with homogenized cercariae produced antibodies against R. fennica. An especially strong response was elicited by infecting fish with living cercariae emerging from infected clams. The specificity of the antibodies, as shown in Western blots, was different between fis…
Ultraviolet B Irradiation Modulates the Immune System of Fish (Rutilus rutilus, Cyprinidae) II: Blood
2007
The effects of a single dose of ultraviolet B (UVB) radiation (0.4 J/cm2) on immunological functions by blood leukocytes and on hematological parameters was studied in roach (Rutilus rutilus), a teleostean fish. The respiratory burst of phorbol 12-myristate 13-acetate stimulated whole blood phagocytes increased significantly after UVB irradiation but spontaneous cytotoxicity of blood leukocytes toward 51chromium-labeled K562 target cells was not markedly altered. Differential cell counting revealed that UVB exposure significantly increased the proportion of granulocytes and significantly decreased the proportion of lymphocytes in the peripheral blood, whereas hematocrit and the total number…
Effects of ultraviolet light on immune parameters of the roach
2000
Ultraviolet B radiation penetrates into water and can affect fish health and the immune system, as is the case with mammals. Teleost fish, the roach, were exposed to UVB irradiation in aquariums and a panel of immune parameters was determined. In addition to altered blood picture and respiratory burst by blood leukocytes, changes were noted also in major lymphatic organs. Respiratory burst and natural cytotoxicity activity of head kidney granulocytes and mitogen-activated proliferation of splenic lymphocytes were suppressed. Although mostly transitory, some parameters remained suppressed for the following 2 weeks. Ultraviolet A radiation had only minor effects. The stress induced by UVB may…
Modulation of immune parameters of roach, Rutilus rutilus, exposed to untreated ECF and TCF bleached pulp effluents
2000
Abstract The present study was designed to assess the effects of elemental chlorine free (ECF) and totally chlorine free (TCF) pulp and paper mill effluents on the immune defence of the roach ( Rutilus rutilus ). Fish were exposed for 5 weeks to concentrations of 0, 0.6, 2, 6 and 20% of untreated effluents from ECF or TCF bleaching processes. In order to study the capability to respond to foreign antigens the fish were immunised with bovine γ-globulin (BGG) 3 weeks before sampling. The numbers of anti-BGG antibody-secreting cells and immunoglobulin-secreting cells in the spleen and blood, as well as the levels of the anti-BGG antibodies and concentrations of immunoglobulin in plasma and the…
Effects of primary- and secondary-treated bleached kraft mill effluents on the immune system and physiological parameters of roach.
2000
The present study was designed to examine, whether, effluents from a modern pulp and paper mill using elemental chlorine-free/total chlorine-free (ECF/TCF) bleaching, exert effects on the immune system of fish and, in addition, to relate these findings to physiological parameters known to be affected by bleached kraft-mill effluents (BKME). Roach (Rutilus rutilus) were exposed in laboratory conditions to primary- or secondary-treated effluent from a pulp and paper mill. In order to study their capability to respond to foreign antigens they were immunised with bovine gamma-globulin (BGG) prior to exposure. The number of anti-BGG antibody-secreting cells (ASC) and the number of immunoglobulin…