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RESEARCH PRODUCT
ATR-Spin: an open-source 3D printed device for direct cytocentrifugation onto attenuated total reflectance crystals.
Diana E. BedollaBayden R. WoodHugh J. ByrneDavid Perez-guaitaDavid Perez-guaitaGuillermo QuintásJulia KuligowskiZack Richardsonsubject
3d printedMaterials scienceSpectrophotometry Infraredbusiness.industryCell preparation010401 analytical chemistryBiomedical EngineeringInfrared spectroscopyBioengineeringCell analysis02 engineering and technologyGeneral Chemistry021001 nanoscience & nanotechnology01 natural sciencesBiochemistry0104 chemical sciencesOpen sourceMolecular levelAttenuated total reflectionPrinting Three-DimensionalSpectroscopy Fourier Transform InfraredOptoelectronics0210 nano-technologybusinessdescription
Infrared Spectroscopy (IR) enables the direct and rapid characterization of cells at the molecular level. Achieving a rapid and consistent cell preparation is critical for the development of Point-of-Care diagnostics for cell analysis. Here we introduce an open-source, 3D printed device for integrating the isolation, preconcentration, and measurement of attenuated total reflectance IR spectra of cells from biofluids. The tool comprises a disposable card for cytocentrifugation, equipped with magnets, which allows reproducible integration into the pathlength of the IR spectrophotometer. Preliminary results using cell media containing A459 cells indicate that this system enables a qualitative and quantitative characterization of cells down to 10 cells µL-1 by using a single and cost-effective device and within a few minutes.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2021-11-26 | Lab on a chip |