6533b7dcfe1ef96bd1271eaa
RESEARCH PRODUCT
In vitro plant regeneration of caper (Capparis spinosa L.) from floral explants and genetic stability of regenerants
Maurizio SajevaMirko SiragusaFrancesco SottileFrancesco CarimiAngela CarraLoredana Abbatesubject
chemistry.chemical_classificationCapparis spinosa L.Floral explant Genetic fidelity Plant regeneration Somaclonal variabilityCapparis spinosafungiStamenfood and beveragesHorticultureBiologyfood.foodSettore AGR/03 - Arboricoltura Generale E Coltivazioni Arboreechemistry.chemical_compoundMurashige and Skoog mediumfoodchemistryMicropropagationAuxinSettore BIO/03 - Botanica Ambientale E ApplicataCytokininBotanyShootSettore BIO/04 - Fisiologia VegetaleExplant culturedescription
A new technique to regenerate caper plants (Capparis spinosa L. subsp. rupestris) starting from flower explant is reported. In vitro plant regeneration was attempted using stigma, anthers and unfertilized ovules of unopened flowers collected in the field. Plant regeneration was achieved from unfertilized ovules on MS medium supplemented with 88 mM sucrose and 13 lM 6-benzyladenine (BA). New individuals obtained from unfertilized ovules were used as source material for micropropagation and multiple shoots were obtained on MS medium sup- plemented with the adeninic cytokinin BA and the auxin indole-3-butyric acid (IBA). Explants obtained in micro- propagation step were used for rooting step under several treatments. The best results (100% of rooted explants) were obtained when explants were dipped for 10 min in 50 lM IBA solution and successively maintained in growth regulator free medium. New plants were vigorous, of good quality and presented phenotypic characters similar to mother plants. Furthermore genetic stability of regenerants was verified through flow cytometric analysis and two different DNA-based techniques.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2011-12-30 | Plant Cell, Tissue and Organ Culture (PCTOC) |