6533b7dcfe1ef96bd1272cb4
RESEARCH PRODUCT
VISMapper: ultra-fast exhaustive cartography of viral insertion sites for gene therapy
José M. JuanesIgnacio MedinaJoaquín DopazoJoaquín TárragaVicente ArnauAsunción GallegoPablo Marin-garciaFelipe J. Chavessubject
0301 basic medicineWeb serverVirus IntegrationGenetic enhancementGenetic VectorsContext (language use)Computational biologyBiologyGenoma humàlcsh:Computer applications to medicine. Medical informaticscomputer.software_genreBiochemistryGenome viewerViral vectorViral integrationUser-Computer Interface03 medical and health sciencesGene therapyStructural BiologySAFERViral insertionSequence mappingHumansUltra fastGens Mapatgelcsh:QH301-705.5Molecular BiologyGeneticsInternetBase SequenceApplied MathematicsHigh-Throughput Nucleotide SequencingGenetic Therapy3. Good healthComputer Science Applications030104 developmental biologylcsh:Biology (General)lcsh:R858-859.7Viral integrationDNA microarraycomputerSoftwaredescription
The possibility of integrating viral vectors to become a persistent part of the host genome makes them a crucial element of clinical gene therapy. However, viral integration has associated risks, such as the unintentional activation of oncogenes that can result in cancer. Therefore, the analysis of integration sites of retroviral vectors is a crucial step in developing safer vectors for therapeutic use. Here we present VISMapper, a vector integration site analysis web server, to analyze next-generation sequencing data for retroviral vector integration sites. VISMapper can be found at: http://vismapper.babelomics.org . Because it uses novel mapping algorithms VISMapper is remarkably faster than previous available programs. It also provides a useful graphical interface to analyze the integration sites found in the genomic context.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-09-01 |