Phenotypic and functional characterization of neutrophils and monocytes from patients with myelodysplastic syndrome by flow cytometry.

6533b7dcfe1ef96bd1273558

RESEARCH PRODUCT

Phenotypic and functional characterization of neutrophils and monocytes from patients with myelodysplastic syndrome by flow cytometry.

Matthias Theobald Jörn F. Dopheide Pamela Aranda Lopez Evi Lauinger-lörsch Cornelia S. Schmidt Markus P. Radsak Hansjörg Schild Frank Schmidt Florian Nolte

subject

0301 basic medicine Male Cell Degranulation Neutrophils Immunology Cell Separation Biology Granulopoiesis Cell Degranulation Monocytes Flow cytometry Immunophenotyping 03 medical and health sciences 0302 clinical medicine Monitoring Immunologic medicine Humans 610 Medicine & health Aged Monitoring Physiologic CD64 Aged 80 and over CD11b Antigen medicine.diagnostic_test Myelodysplastic syndromes Monocyte Receptors IgG Degranulation hemic and immune systems Middle Aged medicine.disease Flow Cytometry Prognosis 030104 developmental biology medicine.anatomical_structure International Prognostic Scoring System Myelodysplastic Syndromes Immunology Female 030215 immunology

description

Myelodysplastic syndrome (MDS) is a clonal stem cell disorder frequently associated with inefficient granulopoiesis showing dysplastic polymorphonuclear neutrophils (PMNs). To assess PMN functionality in MDS in a clinical routine setting, 30 MDS patients and ten healthy volunteers were analyzed for PMN and monocyte phenotype and function (degranulation, CD62L shedding, oxidative burst and phagocytosis) upon stimulation with lipopolysaccharide by multi-color flow cytometry (MCFC). Our data show a heterogeneous pattern for CD66, CD16 and CD64 expression on PMNs of MDS patients. CD62L shedding rate and CD66 degranulation were reduced. Interestingly, we detected correlations between the WHO adapted prognostic scoring system (WPSS) and CD16 expression on PMNs as well as the international prognostic scoring system (IPSS) and CD11b degranulation by MCFC, suggesting clinical relevance of MCFC based function testing. In conclusion, MCFC of myelodysplastic immunophenotypes and PMN functionality are applicable in clinical settings, but further prospective studies are needed to assess the practical clinical value of such analyses.

year	journal	country	edition	language
2016-03-03	Cellular immunology

10.1016/j.cellimm.2016.07.005 https://pubmed.ncbi.nlm.nih.gov/27417453