6533b7ddfe1ef96bd1273e5f

RESEARCH PRODUCT

Carbonyl metallo immuno assay: a new application for Fourier transform infrared spectroscopy

Pierre BrossierGérard JaouenAnne VessièresMichèle Salmain

subject

ImmunoassayDetection limitChromatographymedicine.diagnostic_testChemistryClinical BiochemistryPharmaceutical ScienceInfrared spectroscopyMetal carbonylCombinatorial chemistryAnalytical Chemistrysymbols.namesakeFourier transformImmunoassaySpectroscopy Fourier Transform InfraredDrug DiscoverymedicinesymbolsAnticonvulsantsFourier transform infrared spectroscopyAbsorption (electromagnetic radiation)Quantitative analysis (chemistry)Spectroscopy

description

Abstract We describe here the development of a new, non-isotopic immunological assay termed CMIA (carbonyl metallo immunoassay) that uses metal carbonyl complexes as tracers and Fourier transform infrared spectroscopy (FT-IR) as the detection method. This assay is based on the particular spectral features of these complexes, which show very strong absorption bands in the 1800–2200 cm −1 spectral range where proteins and organic molecules do not absorb. In Section 1 , the optimisation of the quantitative detection of these tracers is detailed. In Section 2 , the implementation of mono-CMIA is described, including the CMIA assays of three antiepileptic drugs (carbamazepine, phenobarbital, phenytoin). Finally, extension to the simultaneous double- and triple-CMIA of these drugs is reported.

yearjournalcountryeditionlanguage
2000-03-04Journal of Pharmaceutical and Biomedical Analysis
https://doi.org/10.1016/s0731-7085(99)00169-7