6533b7ddfe1ef96bd1273f04
RESEARCH PRODUCT
Red blood cell plasmalogens and docosahexaenoic acid are independently reduced in primary open-angle glaucoma
Lionel BretillonPierre JuanédaNiyazi AcarOlivier BerdeauxCatherine Creuzot-garcherStéphane GrégoireCorinne JoffreStéphanie CabaretAlain M. Bronsubject
Malegenetic structuresGlaucomaPathogenesischemistry.chemical_compound0302 clinical medicine[SDV.IDA]Life Sciences [q-bio]/Food engineeringETHER-LIPIDSERYTHROCITEchemistry.chemical_classificationAged 80 and over0303 health sciencesMiddle AgedSensory Systemsmedicine.anatomical_structureBiochemistryDocosahexaenoic acidDisease ProgressionFemalelipids (amino acids peptides and proteins)Glaucoma Open-AnglePolyunsaturated fatty acidmedicine.medical_specialtySpectrometry Mass Electrospray IonizationOpen angle glaucomaDocosahexaenoic AcidsPhospholipidBiology03 medical and health sciencesCellular and Molecular NeuroscienceInternal medicinemedicineHumans[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process EngineeringPLASMALOGENSPRIMARY OPEN-ANGLE GLAUCOMA (POGA)030304 developmental biologyAgedErythrocyte MembraneBlood flowDOCOSAHEXAENOIC ACID (DHA)medicine.diseaseeye diseasesOphthalmologyRed blood cellEndocrinologychemistryCase-Control Studies030221 ophthalmology & optometrysense organsVisual FieldsChromatography Liquiddescription
International audience; Among several theories involved in the pathogenesis of primary open-angle glaucoma (POAG), the vascular theory considers the disease to be a consequence of reduced ocular blood flow associated with red blood cell abnormalities. Red blood cell membrane structure and function are influenced by their phospholipid composition. We investigated whether specific lipid entities that may affect the membrane physiology, namely, polyunsaturated fatty acids (PUFAs) and plasmalogens, are modified in POAG and whether these potential variations are related to the stage of glaucoma. Blood samples were collected from 31 POAG patients and 10 healthy individuals. The stage of glaucoma was determined according to the Hodapp and Parrish classification. Lipids were extracted from red blood cell membranes and individual phospholipid species were quantified by liquid chromatography combined with mass spectrometry using triple quadrupole technology. POAG patients had reduced erythrocyte levels of phosphatidyl-choline (PC) carrying docosahexaenoic acid (DHA). POAG patients also displayed lower levels of choline plasmalogens (PlsC) carrying PUFAs other than DHA. These differences were greater as the severity of the disease increased. Linear regressions predicted that red blood cell PlsC levels would decrease years before clinical symptoms, whereas the levels of PC carrying DHA were linearly correlated to visual field loss. Our data demonstrate the selective loss of some individual phospholipid species in red blood cell membranes, which may partly explain their loss of flexibility in POAG.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2009-01-01 |