6533b7ddfe1ef96bd1274077
RESEARCH PRODUCT
Multisite field potential recordings and analysis of the impulse propagation pattern in cardiac cells culture
Jean-marie BilbaultPierre AthiasGabriel LaurentSabir JacquirMatthieu RosseDavid VandrouxStéphane Binczaksubject
0303 health sciencesMaterials sciencemedicine.diagnostic_test[SDV]Life Sciences [q-bio]Propagation patternTime lagDiscontinuous conduction030204 cardiovascular system & hematologyImpulse (physics)03 medical and health sciencesMicroelectrode[SPI]Engineering Sciences [physics]0302 clinical medicineCellular resolutionmedicineMyocyte[NLIN]Nonlinear Sciences [physics]ElectrocardiographyComputingMilieux_MISCELLANEOUS030304 developmental biologyBiomedical engineeringdescription
To provide further insights into the impulse propagation between cardiac myocytes, we performed multiparametric studies of excitation spread with cellular resolution in confluent monolayers of cultured cardiomyocytes (CM). Simultaneous paired intracellular recordings of action potentials in two individual CM revealed slight periodic spontaneous advances/delays in the interspike time lag. Multisite field potential recordings performed with microelectrode arrays (MEA) confirmed random and iterative cycle-to-cycle changes in the direction of excitation spread. These local spontaneous variations in the cardiac impulse propagation pathways may be a safety process protecting against microscopically discontinuous conduction, and abnormality of this natural process could contribute to the genesis of some heart arrhythmias.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2007-09-30 |