0000000000077753
AUTHOR
Stéphane Binczak
Automatic Processing Scheme for Low Laser Invasiveness Electro Optical Frequency Mapping mode
International audience; Electro optical techniques are efficient backside contactless techniques usually used for design debug and defect location in modern VLSI. Unfortunately, the signal to noise ratio is quite low and depends on laser power with potential device stress due to long acquisition time or high laser power, especially in up to date technologies. Under these conditions, to maintain a good signal or image quality, specific signal or image processing techniques can be implemented. In this paper, we proposed a new spatial filtering by stationary wavelets and contrast enhancement which allows the use of low laser power and short acquisition time in image mode.
Frequency mapping in dynamic light emission with wavelet transform
International audience; Dynamic photon emission microscopy is an e cient tool to analyse today's integrated circuit. Nevertheless, the reduction of transistor's dimensions leads to more complex acquisitions where many spots can be seen. A frequency characterization of the whole acquired area can help to have a better understanding of it. With that purpose in mind, a new methodology to draw frequency mapping of dynamic light emission acquisition is reported. It is fully automated and based on wavelet transform and autocorrelation function. Regarding the possible use in an industrial context, the suggested method can help to localize abnormal emission activity and it gives some perspectives o…
Automatic emission spots identification in static and dynamic imaging by research of local maxima.
International audience
Spatial correction in dynamic photon emission by affine transformation matrix estimation
International audience; Photon emission microscopy and Time Resolved Imaging have proved their efficiency for defect localization on VLSI. A common process to find defect candidate locations is to draw a comparison between acquisitions on a normally working device and a faulty one. In order to be accurate and meaningful, this method requires that the acquisition scene remains the same between the two parts. In practice, it can be difficult to set. In this paper, a method to correct position by affine matrix transformation is suggested. It is based on image features detection, description and matching and affine transformation estimation.
Arrhythmia due to mild therapeutic hypothermia - a study in vitro
International audience; Introduction: In case of resuscitation after CA, the brain suffers the ischemia and the inflammation from reperfusion. To days, the only therapy available is the mild therapeutic hypothermia (MTH) : put the patient under 34°C-32°C during 12-24 hours. MTH has been shown to increase the hospital survival rate, but it has many adverse effects, among which the cardiac arrhythmia generation represents an important part (up to 34%). Cardiac culture in vitro provides a better spatial resolution than study in vivo, which could bring some insights of the mechanism of post-hypothermia arrhythmia generation. Method: Monolayer cardiac culture is prepared with cardiomyocytes from…
Estimation of Muscular Fatigue under Electromyostimulation Using CWT
International audience; The aims of this study are to investigate muscular fatigue and to propose a new fatigue index based on the continuous wavelet transform (CWT) which is compared to the standard fatigue indexes from literature. Fatigue indexes are all based on the electrical activity of muscles (electromyogram) acquired during an electrically stimulated contraction thanks to two modules (electromyostimulation + electromyography recording) that can analyze EMG signals in real time during electromyostimulation. The extracted parameters are compared with each other and their sensitivity to noise is studied. The effect of truncation of M waves is then investigated, enlightening the robustn…
Polymorphic and regular localized activity structures in a two-dimensional two-component reaction–diffusion lattice with complex threshold excitation
Abstract Space–time dynamics of the system modeling collective behaviour of electrically coupled nonlinear units is investigated. The dynamics of a local cell is described by the FitzHugh–Nagumo system with complex threshold excitation. It is shown that such a system supports formation of two distinct kinds of stable two-dimensional spatially localized moving structures without any external stabilizing actions. These are regular and polymorphic structures. The regular structures preserve their shape and velocity under propagation while the shape and velocity as well as other integral characteristics of polymorphic structures show rather complex temporal behaviour. Both kinds of structures r…
Diffusion effects in a nonlinear electrical lattice
International audience; We consider a nonlinear electrical network modeling the generalized Nagumo equation. Focusing on the particular case where the initial load of the lattice consists in the superimposition of a coherent information weakly varying in space and a perturbation of small amplitude, we show that the perturbation can be eliminated quickly, almost without disturbing the information.
Spiking patterns emerging from wave instabilities in a one-dimensional neural lattice.
The dynamics of a one-dimensional lattice (chain) of electrically coupled neurons modeled by the FitzHugh-Nagumo excitable system with modified nonlinearity is investigated. We have found that for certain conditions the lattice exhibits a countable set of pulselike wave solutions. The analysis of homoclinic and heteroclinic bifurcations is given. Corresponding bifurcation sets have the shapes of spirals twisting to the same center. The appearance of chaotic spiking patterns emerging from wave instabilities is discussed.
Multi axis representation and Euclidean distance of muscle fatigue indexes during evoked contractions
International audience; In this article, we proposed a new representation of muscular fatigue during evoked muscle contractions based on fatigue indexes such as peak to peak amplitude, RMS of the M wave, mean and median frequency and fatigue index calculated from continuous wavelet transform (I CWT). These new representations of muscle fatigue using multi axis represented and Euclidean distance give better insights on changes in physiological characteristics during muscle fatigue. This technique provides a fatigue index using several muscle characteristics. The use of other kinds of fatigue characteristics as force could also be possible.
Ephaptic coupling of myelinated nerve fibers
Numerical predictions of a simple myelinated nerve fiber model are compared with theoretical results in the continuum and discrete limits, clarifying the nature of the conduction process on an isolated nerve axon. Since myelinated nerve fibers are often arranged in bundles, this model is used to study ephaptic (nonsynaptic) interactions between impulses on parallel fibers, which may play a functional role in neural processing.
Recurrence quantification analysis as a tool for complex fractionated atrial electrogram discrimination
International audience; Atrial fibrillation is the most encountered pathology of the heart rate. The reasons of its occurrence and its particular characteristics remain unknown, resulting from complex phenomena interaction. From these interactions emerges Complex Fractionated Atrial Electrograms (CFAE) which are useful for the ablation procedure. This study presents a method based on nonlinear data analysis, the Recurrence Quantification Analysis (RQA) applied on intracardiac atrial electrograms to detect CFAE particularities. The results obtained on areas previously tagged by a cardilogist show a good sensitivity to CFAE. Combination of RQA features offers a larger discrimination potential…
Multisite field potential recordings and analysis of the impulse propagation pattern in cardiac cells culture
To provide further insights into the impulse propagation between cardiac myocytes, we performed multiparametric studies of excitation spread with cellular resolution in confluent monolayers of cultured cardiomyocytes (CM). Simultaneous paired intracellular recordings of action potentials in two individual CM revealed slight periodic spontaneous advances/delays in the interspike time lag. Multisite field potential recordings performed with microelectrode arrays (MEA) confirmed random and iterative cycle-to-cycle changes in the direction of excitation spread. These local spontaneous variations in the cardiac impulse propagation pathways may be a safety process protecting against microscopical…
Experimental study of arrhythmia due to mild therapeutic hypothermia after resuscitation of cardiac arrest
International audience; Introduction: One of the important challenges after cardiac arrest (CA) is the neurological damage of the brain. In case of resuscitation after CA, the brain suffers the ischemia and the inflammation from reperfusion. To days, the only therapy available is the mild therapeutic hypothermia (MTH) : put the patient under 34°C-32°C during 12-24 hours. Even though that MTH has been shown to increase the hospital survival rate, it has many adverse effects, among which the cardiac arrhythmia generation represents an important part (up to 34%). Cardiac culture in vitro provides a better spatial resolution (to cellular level) than study in vivo, which could bring some insight…
Experimental study of low-voltage surge protection device response in realistic systems
Experimental results on low-voltage surge protection under fast pulses in realistic wiring systems are presented. A fast voltage pulse generator is designed to provide fast voltage pulses with short steep fronts. The effective residual voltage of protected equipment is then investigated and compared with simulation results.
Experimental study of electrical FitzHugh-Nagumo neurons with modified excitability
International audience; We present an electronical circuit modelling a FitzHugh-Nagumo neuron with a modified excitability. To characterize this basic cell, the bifurcation curves between stability with excitation threshold, bistability and oscillations are investigated. An electrical circuit is then proposed to realize a unidirectional coupling between two cells, mimicking an inter-neuron synaptic coupling. In such a master-slave configuration, we show experimentally how the coupling strength controls the dynamics of the slave neuron, leading to frequency locking, chaotic behavior and synchronization. These phenomena are then studied by phase map analysis. The architecture of a possible ne…
Pattern dynamics in a nonlinear electrical lattice
International audience; In this paper, we present experiments using a nonlinear electrical line, modeling the FitzHugh-Nagumo equation, without recovery term. Different patterns are studied according to the para meters of this medium and initial conditions. We then propose to apply these results to the domain of signal processing. We show that erosion and dilation of a binary signal, two kinds,of binarization-one depending on an amplitude threshold, the other on an energetical threshold-and nonlinear filtering allowing noise removal can be obtained in the same medium.
Implementation of compact VLSI FitzHugh-Nagumo neurons
In this paper we show a low power and very compact VLSI implementation of a FitzHugh-Nagumo neuron for large network implementations. The circuit consists of only 17 small transistors and two capacitors and consumes less than 23 muW. It is composed of a nonlinear resistor and a lossy active inductor. We demonstrate that a simple low Q active inductor can be used instead of a complex one because the parasitic series resistor can be easily embedded to the FitzHugh-Nagumo model. We also perform a statistical analysis to check the robustness of the circuit against mismatch.
Characterization of Radio Frequency Echo using frequency sweeping and power analysis
International audience; Coupling between repeater's antennas, called Radio Frequency Echo (RFE) deteriorates signal quality and compromises system stability. If the channel insulation is insufficient, the power in the closed-loop raises with time according to the gain margin value. Multiple methods of echo cancellation exists. Generally, these techniques are based on correlation calculation and adaptive filtering. However, their efficiency and algorithm's convergence speed are calling into question under signal's modulation and real-time constraints, specially with narrow band signals having frequency hopping as GSM or TETRA. This paper describes a real time method of RFE detection and esti…
Nonlinear mechanics of DNA doule strand: existence of the compact-envelope bright solitary wave
We study the nonlinear dynamics of a homogeneous DNA chain which is based on site-dependent finite stacking and pairing enthalpies. We introduce an extended nonlinear Schrödinger equation describing the dynamics of modulated wave in DNA model. We obtain envelope bright solitary waves with compact support as a solution. Analytical criteria of existence of this solution are derived. The stability of bright compactons is confirmed by numerical simulations of the exact equations of the lattice. The impact of the finite stacking energy is investigated and we show that some of these compact bright solitary waves are robust, while others decompose very quickly depending on the finite stacking para…
Realistic model of compact VLSI FitzHugh–Nagumo oscillators
In this article, we present a compact analogue VLSI implementation of the FitzHugh–Nagumo neuron model, intended to model large-scale, biologically plausible, oscillator networks. As the model requires a series resistor and a parallel capacitor with the inductor, which is the most complex part of the design, it is possible to greatly simplify the active inductor implementation compared to other implementations of this device as typically found in filters by allowing appreciable, but well modelled, nonidealities. We model and obtain the parameters of the inductor nonideal model as an inductance in series with a parasitic resistor and a second order low-pass filter with a large cut-off freque…
Segmentation of Positron Emission Tomography Images Using Multi-atlas Anatomical Magnetic Resonance Imaging (MRI)
Positron emission tomography (PET), is a medical imaging technique, it provides information about the body’s cellular function rather than its anatomy. However, due to the functional nature of PET images, locating the anatomical structures in such an image remains a challenging task, indeed, PET images only provide very little anatomical information. Segmentation of PET images, therefore, requires the intervention of a medical expert. The expert proceeds to a manual segmentation of a volume slice by slice, which turns out to be very tedious and costly in terms of time. In this article, we present, evaluate, and make available a multi-atlas approach for automatically segmenting human brain P…
Spiral wave induced numerically using electrical stimulation and comparison with experimental results.
Experiments in vitro on a Microelectrode Array (MEA) platform show that electrical stimulation can provoke the generation of spiral waves in cardiac tissue. Nevertheless, the conditions leading to this artificial fibrillation state remain unclear. In order to have a better understanding of this phenomenon, a numerical simulation study has been conducted. The results obtained with a two-dimensional FitzHugh-Nagumo model proved that it is possible to create spiral waves by adding a stimulation current under certain conditions, which are made explicit.
Programmable VLSI cubic-like function implementation
An analogue VLSI implementation of a cubic-like function is presented, whose design is focused to reduce the circuit complexity. Simulations show that the V–I characteristic of the circuit resembles a cubic function, which can be easily adjusted by changing the bias parameters.
QUANTIFICATION OF MUSCLE FATIGUE WITHWAVELET ANALYSIS BASED ON EMG DURING MYOELECTRICAL STIMULATION
6; International audience; We propose a device dedicated to real time analysis of electromyograms (EMG) under myoelectrical stimulation (ES). The muscular fatigue analysis, which is obtained by the use of a dedicated analog circuit and a processing part, is the main purpose of this study. The description of a hardware device which incorporates an electro-stimulator and an electromyogram amplifier combined to a computer is detailed. Then, we present a muscular fatigue analysis part based on wavelet decomposition in order to extract a fatigue index, which is confronted with synthetic and experimental data. We conclude that the CWT index applies well to M waves. The noise sensitivity is invest…
Reaction-Diffusion Network For Geometric Multiscale High Speed Image Processing
International audience; In the framework of heavy mid-level processing for high speed imaging, a nonlinear bi-dimensional network is proposed, allowing the implementation of active curve algorithms. Usually this efficient type of algorithm is prohibitive for real-time image processing due to its calculus charge and the inadequate structure for the use of serial or parallel architectures. Another kind of implementation philosophy is proposed here, by considering the active curve generated by a propagation phenomenon inspired from biological modeling. A programmable nonlinear reaction-diffusion system is proposed under front control and technological constraints. Geometric multiscale processin…
A hybrid stimulation strategy for suppression of spiral waves in cardiac tissue
International audience; Atrial fibrillation (AF) is the most common cardiac arrhythmia whose mechanisms are thought to be mainly due to the self perpetuation of spiral waves (SW). To date, available treatment strategies (antiarrhythmic drugs, radiofrequency ablation of the substrate, electrical cardioversion) to restore and to maintain a normal sinus rhythm have limitations and are associated with AF recurrences. The aim of this study was to assess a way of suppressing SW by applying multifocal electrical stimulations in a simulated cardiac tissue using a 2D FitzHugh-Nagumo model specially convenient for AF investigations. We identified stimulation parameters for successful termination of S…
A passive stabilization solution for camera embedded onboard small planes
Embedded camera is becoming democratized in personal uses like sport. Thus the need for a stabilized video leads to a development of dedicated stabilized systems. However, in professional uses like video protection, stabilization solutions are not yet common. Moreover such a system needs to fit application specific requirements in term of size, weight, amplitude and frequency response.
Complexity analysis of experimental cardiac arrhythmia
International audience; To study the cardiac arrhythmia, an in vitro experimental model and Multielectrodes Array (MEA) are used. This platform serves as an intermediary of the electrical activities of cardiac cells and the signal processing / dynamics analysis. Through it the extracellular potential of cardiac cells is acquired, allowing a real-time monitoring / analyzing. Since MEA has 60 electrodes / channels dispatched in a rectangular region, it allows real-time monitoring and signal acquisition on multiple sites. The in vitro experimental model (cardiomyocytes cultures from new-born rats' heart) is directly prepared on the MEA. This carefully prepared culture has similar parameters as…
Noise removal using a nonlinear two-dimensional diffusion network
Un reseau electrique non lineaire bidimensionnel, constitue de N×N cellules identiques, et modelisant l’equation de Nagumo discrete est presente. A l’aide d’une nouvelle description de la fonction non lineaire, on peut predire analytiquement l’evolution temporelle de la partie coherente du signal, ainsi que celle des perturbations de petites amplitudes qui lui sont superposees. Enfin, des applications a l’amelioration du rapport signal sur bruit, ou au traitement d’images sont suggerees.
Controlling muscular force by functional electrical stimulation using intelligent PID
Functional Electrical Stimulation (FES) appears as a way of extremely promising research today to solve numerous pathology connected to the deficiencies of the nervous system. It is usually used for the rehabilitation of people with neurological disorders. Generally, skeletal muscles are activated by using Constant Frequency Trains (CFTs) with a fixed amplitude and inter-pulse duration. In addition, the systems of electrical stimulation do not adapt the parameters stimulation to obtain a desired force response during the rehabilitation session. The purpose of this study is to adapt automatically the stimulation parameters to the force desired by the clinician with an intelligent PID control…
Filtering and emission area identification in the Time Resolved Imaging data
Abstract Time Resolved Imaging (TRI) acquisitions allow precise timing analysis of emission spots. Up to date technologies deeply challenge their isolation by hiding the weak ones, under sizing or over sizing visually detectable emission spots and finally by jeopardizing timing resolution. We report on an algorithm based on 1 and 2D signal processing tools which automates the identification of emission sites and optimizes separation between noise and useful signal, even for weak spots surrounding strong emission areas. The application of the algorithm on several sets of data from different types of devices and their results are also discussed.
A mixed FES/EMG system for real time analysis of muscular fatigue
International audience; In this article, we present a functional electrical stimulator allowing the extraction in real time of M-wave characteristics from resulting EMG recodings in order to quantify muscle fatigue. This system is composed of three parts. A Labview software managing the stimulation output and electromyogram (EMG) input signal, a hardware part amplifying the output and input signal and a link between the two previous parts which is made up from input/output module (NIdaq USB 6251). In order to characterize the fatigue level, the Continuous Wavelet Transform is applied yielding a local maxima detection. The fatigue is represented on a scale from 0 for a fine shaped muscle to …
Compact-envelope bright solitary wave in a DNA double strand
International audience; We study the nonlinear dynamics of a homogeneous DNA chain which is based on site-dependent finite stacking and pairing enthalpies. We introduce an extended nonlinear Schroedinger equation describing the dynamics of modulated waves in DNA model. We obtain envelope bright solitary waves with compact support as a solution. Analytical criteria of existence and stability of this solution are derived. The stability of bright compactons is confirmed by numerical simulations of the exact equations of the lattice. The impact of the fi nite stacking energy is investigated and we show that some of these compact bright solitary waves are very robust, while others decompose quic…
Subpixel determination of imperfect circles characteristics
This article deals with the problem of the determination of characteristics of imperfect circular objects in discrete images, namely the radius and center coordinates. To limit distortion, a multi-level method based on active contours was developed. Its originality is to furnish a set of geometric envelopes in one pass, with a correspondence between grayscale and a regularity scale. The adequacy of this approach was tested with several methods, among them is the Radon-based method. More particularly, this study indicates the relevance of the use of active contours combined with a Radon transform-based method which was improved using a fitting considering the discrete implementation of the R…
A theoretical approach of the propagation through geometrical constraints in cardiac tissue
International audience; The behaviour of impulse propagation in the presence of non-excitable scars and boundaries is a complex phenomenon and induces pathological consequences in cardiac tissue. In this article, a geometrical con¯guration is considered so that cardiac waves propagate through a thin strand, which is connected to a large mass of cells. At this interface, waves can slow down or even be blocked depending on the width of the strand. We present an analytical approach leading to determine the blockade condition, by introducing planar travelling wavefront and circular stationary wave. Eventually, the in°uence of the tissue geometry is examined on the impulse propagation velocity.
Automatic defect localization in VLSI circuits: A fusion approach based on the Dempster-Shafer theory
Defect localization in Very Large Integration Cir-cuits (VLSI) requires to use multi-sensor information such aselectrical waveforms, emission microscopy images and frequencymapping in order to detect, localize and identify the failure. Eachsensor provides a specific kind of feature modeling the evidence.Thus, the defect localization in VLSI can be summarized asa problem of data fusion with heterogeneous and impreciseinformation. This study illustrates how to reproduce the humandecision for modeling and fusing the different multi-sensorfeatures by using the Demspter-Shafer theory. We propose notonly an automatic decision rule for mass functions computingbut also confidence intervals to quantif…
New statistical post processing approach for precise fault and defect localization in TRI database acquired on complex VLSI
International audience; Timing issue, missing or extra state transitions or unusual consumption can be detected and localized by Time Resolved Imaging (TRI) database analysis. Although, long test pattern can challenge this process. The number of photons to process rapidly increases and the acquisition time to have a good signal over noise ratio (SNR) can be prohibitive. As a result, the tracking of the defect emission signature inside a huge database can be quite complicated. In this paper, a method based on data mining techniques is suggested to help the TRI end user to have a good idea about where to start a deeper analysis of the integrated circuit, even with such complex databases.
Model Free Control for muscular force by Functional Electrical Stimulation using pulse width modulation
International audience; Functional Electrical Stimulation (FES) is a useful technique for restoring functions for patients with neurological disorders. Muscle activities can be artificially driven through delivery of electrical pulses to skeletal muscles. Typically, muscles are activated by using constant stimulation train with a fixed parameters (amplitude, frequency and pulse width). In addition, the FES systems do not adapt the parameters stimulation to obtain a desired force response during the rehabilitation session. The purpose of this study is to investigate a real-time FES system for adapting automatically the stimulation parameters (stimulation pulse width) to track a desired force…
Narrowband digital filtering with random frequency hopping spread spectrum
International audience; In digital signal filtering, channels with narrow bandwidth need high order digital filter to be selected without introducing modulation errors. If a carrier randomly switches from a channel to another as in military applications, or some civilian communication standards, it is necessary to detect and estimate these jumps before transposing and analyzing signals in the baseband. This paper presents a real time solution to filter narrow band signals with random frequency hopping spread spectrum. The proposed method is based on three steps. Firstly, the detection of Signal Frequency Hopping (SFH) using the Fast Fourier Transform (FFT), an algorithm to estimate the Domi…
Relationship between complex fractionated atrial electrogram patterns and different heart substrate configurations
International audience; Complex Fractionated Atrial Electrograms (CFAE) were identified as being symptomatic of reentrant activities. Signals recorded by an intracardiac catheter give an imprecise vision of the underlying phenomena that occur in the proarrhythmic substrate. In this study, phenomenological and ionic numerical models were used to simulate a small piece of the atrium substrate. The catheter measurement is also simulated numerically to take into account electrode particularities. Modifications were applied on the numerical substrate. Then stimulation scenarios were generated in the numerical substrate such as planar waves, stable or breaking spirals. Comparison between simulate…
Truncation effects on muscular fatigue indexes based on M waves analysis
International audience; In this paper, we investigate muscular fatigue. We propose a new fatigue index based on the continuous wavelet transform (CWT) and compare it with the standard fatigue indexes from literature. Fatigue indexes are all based on the electrical activity of muscles (electromyogram) acquired during an electrically stimulated contraction (ES). The stimulator and electromyogram system, which were presented in a previous work, allows real-time analysis. The extracted fatigue parameters are compared between each other and their sensitivity to noise is studied. The effect of truncation of M waves is then investigated, enlightening the robustness of the index obtained using CWT.
Pattern image enhancement by extended depth of field
International audience
Cluster matching in time resolved imaging for VLSI analysis
International audience; If scaling has the benefit of enabling manufacturers to design tomorrow's integrated circuits, from the failure analyst point of view it also has the drawback of making devices more complex. The test sequence for modern VLSI can be quite long, with thousands of vector. Dynamic photon emission databases can contain millions of photons representing thousands of state changes in the region of interest. Finding a candidate location where to perform physical analysis is quite challenging, especially if the fault occurs on a single vector. In this paper, we suggest a new methodology to find single vector fault in dynamic photon emission database. The process is applied at …
Cardiac arrhythmia induced by hypothermia in a cardiac model in vitro
nouvelle version avec l'article complet; International audience; The neurological damage after cardiac arrest (CA) constitutes a big challenge of hospital discharge since years. The therapeutic hypothermia therapy (34°C-32°C) has shown its benefit to reduce cerebral oxygen demand and improve neurological outcomes after the cardiac arrest. Despite the fact that induced hypothermia after CA has been shown to increase the hospital survival rate, it can have many adverse effects, among which the cardiac arrhythmia generation represents an important part (up to 34%, according different clinical studies). Compared to studies in vivo, cardiac culture in vitro provides a better spatial resolution a…
EMERGENCE OF TRAVELLING WAVES IN SMOOTH NERVE FIBRES
International audience; An approximate analytical solution characterizing initial condi- tions leading to action potential ¯ring in smooth nerve ¯bres is determined, using the bistable equation. In the ¯rst place, we present a non-trivial sta- tionary solution wave. Then, we extract the main features of this solution to obtain a frontier condition between the initiation of the travelling waves and a decay to the resting state. This frontier corresponds to a separatrix in the projected dynamics diagram depending on the width and the amplitude of the stationary wave.
Analysis of an Experimental Model of In Vitro Cardiac Tissue Using Phase Space Reconstruction
International audience; The in vitro cultures of cardiac cells represent valuable models to study the mechanism of the arrhythmias at the cellular level. But the dynamics of these experimental models cannot be characterized precisely, as they include a lot of parameters that depend on experimental conditions. This paper is devoted to the investigation of the dynamics of an in vitro model using a phase space reconstruction. Our model, based on the heart cells of new born rats, generates electrical field potentials acquired using a microelectrode technology, which are analyzed in normal and under external stimulation conditions. Phase space reconstructions of electrical field potential signal…
EMG artifacts removal during electrical stimulation, a CWT based technique
International audience; A technique of artifacts removal based on the continuous wavelet transform is presented. It uses common mother wavelets to find the temporal localization of stimulation artifacts on electromyogram (EMG) signal during an electrically evoked contraction of a muscle. This method can be used with standard stimulation pulse waveforms like monophasics or biphasics ones. It uses a histogram representation to find the best threshold to apply on the CWT domain. The algotithm is presented with Haar wavelet and then it is used with common wavelet famillies such as Daubechies or Symlets.
A real time electromyostimulator linked with EMG analysis device
International audience; In this study, a new system composed of two modules (electromyostimulation + electromyography recording) is presented. It can analyze in real time EMG signals during electromyostimulation. In addition, we propose a new method based on wavelet decomposition to analyze changes in M-wave characteristics. It leads to introduce a new index related to muscular fatigue.
Cardiac arrhythmias induced by an electrical stimulation at a cellular level
To provide insights into the impulse propagation between cardiac myocytes, we performed studies of excitation spread with cellular resolution in confluent monolayers of cultured cardiomyocytes (CM). Multisite field potentials have been recorded using microelectrode arrays (MEA) technology in a basal condition and in proarrhythmic conditions induced by a high frequency electrical stimulation. The in vitro observation of spiral waves opens a new way to test the anti-arrhythmic drugs or strategies at cellular level.
Heteroclinic contours and self-replicated solitary waves in a reaction–diffusion lattice with complex threshold excitation
Abstract The space–time dynamics of the network system modeling collective behavior of electrically coupled nonlinear cells is investigated. The dynamics of a local cell is described by the FitzHugh–Nagumo system with complex threshold excitation. Heteroclinic orbits defining traveling wave front solutions are investigated in a moving frame system. A heteroclinic contour formed by separatrix manifolds of two saddle-foci is found in the phase space. The existence of such structure indicates the appearance of complex wave patterns in the network. Such solutions have been confirmed and analyzed numerically. Complex homoclinic orbits found in the neighborhood of the heteroclinic contour define …
Parameters analysis of FitzHugh-Nagumo model for a reliable simulation
International audience; Derived from the pioneer ionic Hodgkin-Huxley model and due to its simplicity and richness from a point view of nonlinear dynamics, the FitzHugh-Nagumo model has been one of the most successful neuron / cardiac cell model. It exists many variations of the original FHN model. Though these FHN type models help to enrich the dynamics of the FHN model. The parameters used in these models are often in biased conditions. The related results would be questionable. So, in this study, the aim is to find the parameter thresholds for one of the commonly used FHN model in order to pride a better simulation environment. The results showed at first that inappropriate time step and…
On the control of a muscular force model including muscular fatigue
Electromyostimulation has been used for several decades by athletes or physiotherapists in order to create a muscular reinforcement. However, the efficiency of electromyostimulation is limited by muscular fatigue and by induced pain. Currently, the systems of electromyostimulation do not adapt the stimulation parameters automatically by taking into account physiological parameters such as muscular fatigue. To adapt the stimulation parameters to muscular responses and in order to optimize the rehabilitation sessions, a control of force using an indicator of muscular fatigue could be used. In this paper, we propose two ways to control the force by using a physiological model which includes th…
Comparison of complex fractionated atrial electrograms at cellular scale using numerical and experimental models.
This study investigates the existence of the pseudo complex fractionated atrial electrogram (CFAE) at cellular level. Our assumptions are based on the fact that CFAEs are linked to the generation of the spiral waves. These are created using a numerical model and an experimental model of in vitro culture of neonatal rats cardiac cells. Pseudo bipolar electrograms resulting from these two models are compared qualitatively and some patterns could be identified as CFAE signature.
Pattern image enhancement by extended depth of field
Abstract Most optical defect localization techniques such as dynamic laser stimulation or photon emission microscopy require a pattern image of the device to be taken. The main purpose is for device navigation, but it also enables the analyst to identify the location of the monitored activity by superimposing it onto the pattern image. The defect localization workflow usually starts at low or medium magnification. At these scales, several factors can lead to a lack of orthogonality of the sample with the optical axis of the system. Therefore, images can be locally out of focus and poorly resolved. In this paper, a method based on Depth of Field Extension is suggested to correct the pattern …
Study of fibrosis induced by an implanted medical device
National audience; This paper focuses on the study of fibrosis induced by an implanted medical device and explores the possibility of characterizing this process by in situ measurement of electrical impedance. The approach combines electrical and biological characterizations of fibrotic tissue, applied to electrodes implanted in animal models. A comparative study of electrical and biological parameters collected at the same time will enable the identification of an electrical marker of fibrosis development, which can be used for establishing a monitoring method. Adopting an interdisciplinary approach, intermediate embedded prototypes, autonomous and portable by animals, will be developed to…
Dynamics of a charge transfer through a memristor between two initially charged cells
A charge-controlled memristor model, with the memristive expression M(q) defined as the ratio between the voltage through the memristor and the current flowing into it, is used in the quantitative study of the charge transfer between two cells initially charged with known voltages. Calling q0 the total charge having flown into the memristor since its first use illustrating the memory feature of this component-, we show that the derivative of M(q) versus q must be a continuous function of q, leading us to improve the expression for M(q). Then, the dynamics of this charge transfer, as well as the time evolution of the voltages of both cells, is analyzed analytically and confirmed by numerical…
Experimental nonlinear electrical reaction-diffusion lattice
International audience; A nonlinear electrical reaction-diffusion lattice modelling the Nagumo equation is presented. It is shown that this system supports front propagation with a given velocity. This propagation is observed experimentally using a video acquisition system, and the measured velocity of the front is in perfect agreement with the theoretical prediction.
Anti-phase wave patterns in a ring of electrically coupled oscillatory neurons
International audience; Space-time dynamics of the network system modeling collective behavior of electrically coupled nonlinear cells is investigated. The dynamics of a local cell is described by the dimensionless Morris-Lecar system. It is shown that such a system yields a special class of traveling localized collective activity so called "anti-phase wave patterns". The mechanisms of formation of the patterns are discussed and the region of their existence is obtained by using the weakly coupled oscillators theory.
<title>Reaction-diffusion electrical network for image processing</title>
We consider an experimental setup, modelling the FitzHugh-Nagumo equation without recovery term and composed of a 1D nonlinear electrical network made up of discrete bistable cells, resistively coupled. In the first place, we study the propagation of topological fronts in the continuum limit, then in more discrete case. We propose to apply these results to the domain of signal processing. We show that erosion and dilation of a binary signal, can be obtained. Finally, we extend the study to 2D lattices and show that it can be of great interest in image processing techniques.© (2006) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted fo…
Experimental study of bifurcations in modified FitzHugh-Nagumo cell
A nonlinear electrical circuit is proposed as a basic cell for modelling the FitzHugh-Nagumo equation with a modified excitability. Depending on initial conditions and parameters, experiments show various dynamics including stability with excitation threshold, bistability and oscillations.
Unsupervised image processing scheme for transistor photon emission analysis in order to identify defect location
International audience; The study of the light emitted by transistors in a highly scaled complementary metal oxide semiconductor (CMOS) integrated circuit (IC) has become a key method with which to analyze faulty devices, track the failure root cause, and have candidate locations for where to start the physical analysis. The localization of defective areas in IC corresponds to a reliability check and gives information to the designer to improve the IC design. The scaling of CMOS leads to an increase in the number of active nodes inside the acquisition area. There are also more differences between the spot’s intensities. In order to improve the identification of all of the photon emission sp…
Étude et optimisation d'un système d'annulation d'écho pour répéteur de radiocommunication
International audience; Le phénomène d'écho en radio-fréquence (RF) se produit au sein d'un répéteur iso-fréquence (IF) lorsque l'isolation entre l'antenne d'émission et l'antenne de réception est insuffisante. L'objectif de ces travaux est de trouver un système d'identification et d'annulation d'écho, en se basant sur le traitement avancé du signal. Cette solution doit être implémenatable matériellement, sur un cir- cuit programmable type FPGA, l'objectif est de travailler en temps réel. La vitesse de convergence des algorithmes, leurs coûts en terme de ressources matérielles et leurs robustesses aux bruits font partie de l'optimisation recherchée. Cette solution doit être utilisable pour …
Détection d'Anomalie dans les Signaux Physiologiques
International audience; Les signaux physiologiques sont des séries temporelles riches en informations. Analyser ces signaux pour extraire ces informations, pour établir un diagnostic ou encore pour prédire une évolution, nécessite des outils performants et adaptés à leurs caractéristiques intrinsèques. En effet le comportement d'un système biologique dépend des variations de très nombreux paramètres, ce qui le rend alors presque imprévisible. Les méthodes issues de la théorie du chaos et de la dynamique non linéaire apportent des éléments qui permettent de comprendre ce type de comportements, et d'établir ainsi un lien qualitatif avec des modèles mathématiques bio-inspirés ou phénoménologiq…
Phase Space Reconstruction of an Experimental Cardiac Electrical Signal
International audience; Cardiac arrhythmias are very common pathologies which can be treated either by medications, invasive ablation procedures or device implantations. In order to improve theses treatments, clinical and experimental models are used to test new drugs. In this context, in vitro cultures of cardiac cells represent valuable models to study the mechanism of the arrhythmias at the cellular level. In this paper, we investigate the stability and robustness of an experimental model in normal and under external stimulation conditions. Phase space reconstructions of attractors in normal and arrhythmic cases are performed after characterizing the nonlinearity of the model, computing …
Active spike transmission in the neuron model with a winding threshold manifold
International audience; We analyze spiking responses of excitable neuron model with a winding threshold manifold on a pulse stimulation. The model is stimulated with external pulse stimuli and can generate nonlinear integrate-and-fire and resonant responses typical for excitable neuronal cells (all-or-none). In addition we show that for certain parameter range there is a possibility to trigger a spiking sequence with a finite number of spikes (a spiking message) in the response on a short stimulus pulse. So active transformation of N incoming pulses to M (with M>N) outgoing spikes is possible. At the level of single neuron computations such property can provide an active "spike source" comp…
ANALYTICAL DETERMINATION OF INITIAL CONDITIONS LEADING TO FIRING IN NERVE FIBERS
International audience; An analytical solution characterizing initial conditions leading to action potential firing in smooth nerve fibers is determined, using the bistable equation. In the first place, we present a nontrivial stationary solution wave, then, using the perturbative method, we analyze the stability of this stationary wave. We show that it corresponds to a frontier between the initiation of the travelling waves and a decay to the resting state. Eventually, this analytical approach is extended to FitzHugh-Nagumo model.
EXPERIMENTAL PROPAGATION FAILURE IN A NONLINEAR ELECTRICAL LATTICE
We consider an experimental setup, modeling the FitzHugh–Nagumo equation without recovery term and composed of a nonlinear electrical network made up of discrete bistable cells, resistively coupled. In the first place, we study experimentally the propagation of topological fronts in the continuum limit where the analytical solution can be obtained. We show that experimental results match the theoretical predictions. The discrete case is then investigated theoretically and in the lattice, emphasizing the pinning of traveling waves.
Active spike responses of analog electrical neuron: Theory and experiments
Using an analog electrical FitzHugh-Nagumo neuron including complex threshold excitation (CTE) properties, we analyze its spiking responses under pulse stimulation corresponding to oscillating threshold manifold. The system is subjected to outside pulse stimulus and can generate nonlinear integrate-and-flre and resonant responses which are typical for excitable neuronal cells ("all-or-none"). The answer of the neuron strongly depends on the number and the characteristics of incoming impulses (amplitude, width, strength and frequency). For certain parameters range, there is a possibility to trigger a spiking sequence with a finite number of spikes in response of a single short stimulus pulse…