6533b7ddfe1ef96bd12740ef

RESEARCH PRODUCT

Erythrocyte Membrane Phosphatidylserine Exposure in Obesity

Marcial MartínezFrancisco EspañaAntonio MoscardóEva SoláAntonio Hernández-mijaresDolores CorellaMaria-luisa SantaolariaAmparo Vayá

subject

AdultErythrocyte AggregationMalemedicine.medical_specialtyAdolescentEndocrinology Diabetes and MetabolismMedicine (miscellaneous)Phosphatidylserinesmedicine.disease_causeErythrocyte aggregationFlow cytometrychemistry.chemical_compoundYoung AdultEndocrinologyWeight lossInternal medicineErythrocyte DeformabilityMalondialdehydeWeight LossMedicineErythrocyte deformabilityHumansObesityProtein PrecursorsNutrition and Dieteticsmedicine.diagnostic_testbusiness.industryErythrocyte MembranePhosphatidylserineMiddle AgedMalondialdehydePathophysiologyPeptide FragmentsOxidative StressEndocrinologychemistryCase-Control StudiesImmunologyFemaleProthrombinmedicine.symptombusinessOxidative stress

description

It has been suggested that increased erythrocyte membrane phosphatidylserine (PS) exposure could contribute to hypercoagulability and hemorheological disturbances in obesity. The aim of our study was to evaluate PS exposure in obese patients and in a control group and to correlate this with hemorheological properties, i.e., erythrocyte aggregability (EA) and deformability, and to evaluate the effect of weight loss on these parameters. An anthropometric and analytical evaluation was performed at baseline and after 3 months on a diet (very low-calorie diet for 4 weeks and low-calorie diet for 2 months) on 49 severe or morbid obese patients (37 women, 12 men) and 55 healthy volunteers (39 women, 16 men). PS exposure on erythrocyte membrane was performed by flow cytometry. Erythrocyte aggregation was measured using the Myrenne MA(1) and the Sefam aggregometer. Erythrocyte deformability was determined in a stress diffractometer. Prothrombin fragment F1+2 (F1+2) was determined as a marker of the hypercoagulable state, and plasma malondialdehyde (MDA) as an indicator of oxidative stress. Obese patients had a higher EA index, higher PS exposure on erythrocyte membranes and higher levels of MDA and F1+2. The differences in erythrocyte aggregation and F1+2 between obese patients and the control group were maintained after adjusting for PS exposure. After 3 months of diet, a significant reduction in PS exposure on erythrocyte membrane was observed. Obese patients show increased PS exposure on erythrocyte membrane, with no effect on rheological properties. Increased PS exposure could contribute to hypercoagulability in these patients. Weight loss obtained with diet treatment reduces PS exposure on erythrocyte membrane.

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