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RESEARCH PRODUCT
Self-diploidization in Saccharomyces cerevisiae kar2 heterokaryons
Ana C. AdamJulio PolainaL. Del Castillosubject
GeneticsHeterokaryonMating typeZygoteGenotypebiologyZygoteGenes FungalSaccharomyces cerevisiaeSaccharomyces cerevisiaeGeneral Medicinebiology.organism_classificationDiploidyKaryogamyPhenotypePolyploidKaryotypingMutationGeneticsMatingPloidyCrosses Geneticdescription
Zygotes isolated by micromanipulation from crosses of Saccharomyces cerevisiae strains, one of which carries a kar mutation, give rise most frequently to cytoductant colonies showing the nuclear constitution of either one of the two haploid parental strains. In crosses of kar2-1 strains to wild-type, about 10% of the cytoductants of both mating types are homozygous autodiploids. There is evidence indicating that self-diploidization occurs by fusion between sibling nuclei in the heterokaryotic zygote. Here we describe this phenomenon and propose to take advantage of it for the construction of genotypically-defined diploids able to mate, and of polyploid strains, which are useful tools in genetic and cytological studies.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1993-11-01 | Current Genetics |