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RESEARCH PRODUCT
Tuning the surface of nanoparticles: Impact of poly(2-ethyl-2-oxazoline) on protein adsorption in serum and cellular uptake
Dana WestmeierUlrike BraunThomas LangThomas LangAnnabelle BertinAnnabelle BertinBenjamin G. R. MohrChristian WürthDominic DocterUte Resch-gengerOlga KoshkinaOlga KoshkinaHelmut SchlaadAngelina HahlbrockMurat EravciMichael MaskosRoland H. StauberChristoph WeiseRaphael ThiermannRaphael ThiermannChristoph Bantzsubject
SerumTime FactorsPolymers and PlasticsSurface PropertiesNanoparticleBioengineeringProtein Corona02 engineering and technologyChemical Fractionation010402 general chemistry01 natural sciencesCell LineBiomaterialschemistry.chemical_compoundAdsorptionDynamic light scatteringMaterials ChemistryPolyaminesOrganic chemistryHumanspoly(2-ethyl-2-oxazoline)Particle SizeElectrophoresis Agar Gelpoly(ethylene glycol)RhodaminesProteinscellular uptake021001 nanoscience & nanotechnologyprotein adsorptionDynamic Light ScatteringEndocytosis0104 chemical scienceschemistryChemical engineeringSurface modificationNanomedicineInstitut für ChemienanoparticlesAdsorption0210 nano-technologyEthylene glycolNanomedicine Radboud Institute for Molecular Life Sciences [Radboudumc 19]BiotechnologyProtein adsorptiondescription
Item does not contain fulltext Due to the adsorption of biomolecules, the control of the biodistribution of nanoparticles is still one of the major challenges of nanomedicine. Poly(2-ethyl-2-oxazoline) (PEtOx) for surface modification of nanoparticles is applied and both protein adsorption and cellular uptake of PEtOxylated nanoparticles versus nanoparticles coated with poly(ethylene glycol) (PEG) and non-coated positively and negatively charged nanoparticles are compared. Therefore, fluorescent poly(organosiloxane) nanoparticles of 15 nm radius are synthesized, which are used as a scaffold for surface modification in a grafting onto approach. With multi-angle dynamic light scattering, asymmetrical flow field-flow fractionation, gel electrophoresis, and liquid chromatography-mass spectrometry, it is demonstrated that protein adsorption on PEtOxylated nanoparticles is extremely low, similar as on PEGylated nanoparticles. Moreover, quantitative microscopy reveals that PEtOxylation significantly reduces the non-specific cellular uptake, particularly by macrophage-like cells. Collectively, studies demonstrate that PEtOx is a very effective alternative to PEG for stealth modification of the surface of nanoparticles.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2016-01-01 |